TAILIEUCHUNG - Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.)

The N-termini of the NADPH : protochlorophyllide oxidoreductase (POR) proteins A and B from barley and POR from pea were determined by acet-ylation of the proteins and selective isolation of the N-terminal peptides for mass spectrometry de novosequence analysis. We show that the cleav-age sites between the transit peptides and the three mature POR proteins are homologous. | ễFEBS Journal Identification of the N-termini of NADPH protochlorophyllide oxidoreductase A and B from barley etioplasts Hordeum vulgare L. Matthias Ploscher1 Bernhard Granvogl1 Veronika Reisinger1 and Lutz A. Eichacker2 1 Department of Biology I Ludwig-Maximilians-University Munich Germany 2 Center for Organelle Research CORE Universitetet i Stavanger Norway Keywords etioplast N-terminus PORA protochlorophyllide oxidoreductase transit peptide Correspondence L. A. Eichacker Center for Organelle Research CORE Universitetet I Stavanger Kristine Bonnevis vei 22 N-4036 Stavanger Norway Fax 47 518 31860 Tel 47 518 31896 E-mail Received 11 October 2008 revised 7 December 2008 accepted 10 December 2008 The N-termini of the NADPH protochlorophyllide oxidoreductase POR proteins A and B from barley and POR from pea were determined by acetylation of the proteins and selective isolation of the N-terminal peptides for mass spectrometry de novo sequence analysis. We show that the cleavage sites between the transit peptides and the three mature POR proteins are homologous. The N-terminus in PORA is V48 that in PORB is A61 and that in POR from pea is E64. For the PORB protein two additional N-termini were identified as A62 and A63 with decreased signal intensity of the corresponding N-terminal peptides. The results show that the transit peptide of PORA is considerably shorter than previously reported and predicted by ChloroP. A pentapeptide motif that has been characterized as responsible for binding of protochlorophyllide to the transit peptide of PORA Reinbothe C Pollmann S Phetsarath-Faure P Quigley F Weis-beek P Reinbothe S 2008 Plant Physiol 148 694-703 is shown here to be part of the mature PORA protein. doi The first step of plant greening is catalysed by NADPH protochlorophyllide oxidoreductase POR which is one of the most abundant enzymes found in etioplasts. The enzyme catalyses the light-activated reduction of .

• Báo cáo khoa học
• medical reports
• thesis report
• traditional medicine
• medical knowledge
• medical research
• analysis of cytoplasmic
• statements and documents scientific reports
• clinical trials
• medical management
• health care
• medical transfer
• the developing countries