TAILIEUCHUNG - Handbook of Microbiological Media, Fourth Edition part 13

Handbook of Microbiological Media, Fourth Edition part 13 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Ancalomicrobium adetum Medium 115 Yeast pH at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Distribute into tubes or flasks using anaerobic techniques and 100 CO2 as gas phase. Use For the cultivation of Anaerospirillum succiniciproducens. Anaerovibrio burkinabensis Medium Composition per Solution Solution Solution Solution E Vitamin solution . Solution Solution Solution B Trace elements solution SL-10 . pH at 25 C Solution A Composition per Preparation of Solution A Add components to distilled deionized water and bring volume to . Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3-4 min. Allow to cool to room temperature while gassing under 80 N2 20 CO2. Continue gassing until pH reaches below . Seal the flask under 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Solution B Trace Elements Solution SL-10 Composition per liter HCl 25 solution . Preparation of Solution B Trace Elements Solution SL-10 Add FeCl2-4H2O to of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to . Add remaining components. Mix thoroughly. Gas under 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Solution C Composition per Preparation of Solution C Add NaHCO3 to distilled deionized water and bring volume to . Mix thoroughly. Filter sterilize. Gas under 80 N2 20 CO2. Solution D Composition per Sodium Preparation of Solution D Add

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