TAILIEUCHUNG - Báo cáo khoa học: Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch

Saccharomyces cerevisiaecells control their cell size at a point in late G1 called Start. Here, we describe a negative role for the Sin3⁄Rpd3 histone deacetylase complex in the regulation of cell size at Start. Initiation of G1 ⁄S-specific transcription ofCLN1, CLN2andPCL1in a sin3Dstrain occurs at a reduced cell size compared with a wild-type strain. | Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch Department of Biology Friedrich-Alexander-University Erlangen-Nurnberg Germany Keywords G1 cyclins histone deacetylase Rpd3 Swi4 Swi6 Correspondence C. Koch Department of Biology Chair for Biochemistry Friedrich-Alexander-University Erlangen-Nurnberg Staudtstr. 5 91058 Erlangen Germany Fax 49 9131 8528254 Tel 49 9131 8528257 E-mail ckoch@ Received 21 February 2009 revised 7 May 2009 accepted 13 May 2009 doi Saccharomyces cerevisiae cells control their cell size at a point in late G1 called Start. Here we describe a negative role for the Sin3 Rpd3 histone deacetylase complex in the regulation of cell size at Start. Initiation of Gi S-specific transcription of CLN1 CLN2 and PCL1 in a sin3D strain occurs at a reduced cell size compared with a wild-type strain. In addition inactivation of the transcriptional regulator SIN3 partially suppressed a cln3D mutant causing sin3Dcln3D double mutants to start the cell cycle at wild-type size. Chromatin immunoprecipitation results demonstrate that Sin3 and Rpd3 are recruited to promoters of SBF Swi4 Swi6 -regulated genes and reveal that binding of Sin3 to SBF-specific promoters is cellcycle regulated. We observe that transcriptional repression of SBF-depen-dent genes in early Gi coincides with the recruitment of Sin3 to specific promoters whereas binding of Sin3 is abolished from Swi4 Swi6-regulated promoters when transcription is activated at the Gi to S phase transition. We conclude that the Sin3 Rpd3 histone deacetylase complex helps to prevent premature activation of the S phase in daughter cells. Introduction Most eucaryotic cells regulate their commitment to cell division at the Gi to S phase transition. In the budding yeast Saccharomyces cerevisiae the events in late Gi leading to S-phase entry are collectively referred to as Start 1-3 . During the G1 phase

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