TAILIEUCHUNG - Báo cáo khoa học: Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain (1,3⁄1,6)-b-D-glucans, galacto- and rhamnomannans, (1,3;1,4)-b-D-glucans and chitin

A procedure has been developed for the isolation of cell walls from the hyphae of the causal agent for barley leaf scald,Rhynchosporium secalis (Oudem) . Davis. Based primarily on monosaccharide linkage analysis, but also on the limited use of linkage-specific glucan hydrolases and solvent fractionation, the walls consist predominantly of (1,3⁄1,6)-b-d-glucans | Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain 1 3 1 6 -p-D-glucans galacto- and rhamnomannans 1 3 1 4 -p-D-glucans and chitin Filomena Pettolino1 Izumi Sasaki2 z Alisa Turbic1 Sarah M. Wilson1 Antony Bacic1 Maria Hrmova3 and Geoffrey B. Fincher3 1 Schoolof Botany University of Melbourne Parkville Australia 2 Department of Materials Chemistry and Bioengineering Oyama NationalCollege of Technology Japan 3 Schoolof Agriculture Food and Wine and the Australian Centre for Plant FunctionalGenomics University of Adelaide Australia Keywords barley leaf scald cellwallpolysaccharides electron microscopy pathogenesis wall layers Correspondence G. B. Fincher Schoolof Agriculture Food and Wine and the Australian Centre for Plant FunctionalGenomics University of Adelaide Waite Campus Glen Osmond South Australia 5064 Australia Fax 61 8 8303 7102 Tel 61 8 8303 7296 E-mail These authors contributed equally to this work Received 30 January 2009 revised 6 April 2009 accepted 6 May 2009 doi A procedure has been developed for the isolation of cell walls from the hyphae of the causal agent for barley leaf scald Rhynchosporium secalis Oudem . Davis. Based primarily on monosaccharide linkage analysis but also on the limited use of linkage-specific glucan hydrolases and solvent fractionation the walls consist predominantly of 1 3 1 6 -P-D-glucans 1 3 1 4 -P-D-glucans galactomannans of 1 2 1 6 -Manp residues and 1 5 -galactofuranosyl 1 5 -Galf side chains rhamnomannans of 1 6 -Manp residues and rhamnopyranosyl 1 2 -Rhap side chains and chitin the walls also contain 23 w w protein. Electron microscopy shows the presence of distinct inner and outer wall layers. Treatment of wall preparations with guanidine hydrochloride dissolves the outer layer and enables separate analysis of the inner and outer walls. The insoluble inner wall layer is composed of 1 3 1 6 -P-D-glucans galacto- and rhamnomannans 1 3

• Báo cáo khoa học
• medical report
• Scientific reports
• scientific research
• intermediate cells
• cell proliferation
• medical analysis
• thesis report
• medical knowledge
• medical research
• analysis of cytoplasmic
• Nobel Prizes
• health technologies
• medical ebook
• medical document
• medical specific study
• tumor cells
• chemical reaction
• Crystal structure
• biochemical studies
• cytoplasm