TAILIEUCHUNG - Báo cáo sinh học: "Chromosome analysis of horse oocytes cultured in vitro"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Chromosome analysis of horse oocytes cultured in vitro | . L-WO Genet Sei Evol 1990 22 151-160 Elsevier INRA 151 Original article Chromosome analysis of horse oocytes cultured in vitro WA King1 M Desjardins2 KP Xu1 D Bousquet3 1OVC University of Guelph Department of Biomedical Sciences sovc University of Guelph Department of Clinical Studies Guelph Ontario NIG 2W1 Canada 3Centre d insémination artificielle du Québec Inc 3456 Sicotte St-Hyacinthe Quebec J2S 2M2 Canada Received 23 October 1989 accepted 22 February 1990 Summary - Oocytes collected from slaughtered mares of unknown reproductive history were cultured in modified Krebs Ringers bicarbonate supplemented with fetal calf serum 20 and fixed for chromosome analysis. To determine the time required for nuclear maturation oocytes were fixed either after 12 h n 21 24 h n 21 48 h n 20 and 60-96 h n 12 or without culture n 30 . In all 89 of those suitable for analysis meiosis was resumed with 59 reaching second metaphase Mil stage. In the majority of oocytes germinal vesicle breakdown occurred by the end of the 1st 12 h of culture and Mil was reached by 24 h. To examine the chromosome features an additional 113 oocytecumulus-complexes were cultured for 24 h before fixation. In all 7 diakinesis metaphàse I MI and 36 Mil spreads could be analyzed. Of the Mil spreads 5 were found to be lacking chromosomes 1 had an excess of chromosomes and 1 was diploid. Compensating for possible artifactual loss of chromosomes the rate of non-disjunction or anaphase lagging was calculated to be It was concluded that with respect to timing and chromosomal features nuclear maturation of in vitro cultured oocytes in horses resembles that of other domestic animals. meiosis oocyte nuclear-maturation non-disjunction horse Résumé Analyse des chromosomes d ovocytes équins cultivés in vitro. Les ovocytes équins utilisés lors de cette étude furent prélẽvés chez des ovaires de juments ay ant un statut reproductif inconnu. Ces ovocytes furent cultivés dans une solution de .

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