TAILIEUCHUNG - Báo cáo sinh học: "B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis | Almeida et al. Genetic Vaccines and Therapy 2011 9 5 http content 9 1 5 GENETIC VACCINES AND THERAPY RESEARCH Open Access B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis 1 11 11 Luciana P Almeida Ana PF Trombone Julio CC Lorenzi Carolina D Rocha Thiago Malardo 1111 2 Isabela C Fontoura Ana F Gembre Ricardo LL Silva Célio L Silva Ademilson P Castelo Arlete AM Coelho-Castelo1 Abstract Background Although B cells are important as antigen presenting cells APC during the immune response their role in DNA vaccination models is unknown. Methods In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. Results In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein pcDNA3-Hsp65 internalization and protect B knock-out BKO mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. Conclusions These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model. Background DNA vaccines consist of the specific gene of interest cloned into a bacterial plasmid that is engineered for optimal expression in eukaryotic cells thereby leading to protective immune responses. In contrast with subunit vaccines in DNA vaccination the immunogen is synthesized within the host by cells that have taken up the antigen-encoding DNA. Consequently DNA vaccines join the advantages of subunit vaccines with the ability to generate antigens endogenously making them accessible to presentation. Moreover CpG motifs in bacterial DNA can elicit innate immune response via Toll-like receptor 9-dependent .

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