TAILIEUCHUNG - Báo cáo sinh học: "Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer | Genetic Vaccines and Therapy BioMed Central Short paper Open Access Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer Srinivas Nagarajh Carsten Ziskef and Ingo GH Schmidt-Wolf Address Department of Internal Medicine I General Internal Medicine Rheinische Friedrich-Wilhelms-Universitat Bonn Germany Email Srinivas Nagaraj- srinivas@ Carsten Ziske - Ingo GH Schmidt-Wolf - Wolf@ Corresponding author fEqual contributors Published 25 August 2004 Received II February 2004 Genetic Vaccines and Therapy 2004 2 12 doi 1479-0556-2-12 Accepted 25 August 2004 This article is available from http content 2 1 12 2004 Nagaraj et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Modulation of the immune system by genetically modified immunological effector cells is of potential therapeutic value in the treatment of malignancies. Interleukin-2 IL-2 is a crucial cytokine which induces potent antitumor response. Cytokine-induced killer cells CIK have been described as highly efficient cytotoxic effector cells capable of lysing tumor cell targets and are capable of recognizing these cells in a non-MHC restricted fashion. Dendritic cells DC are the major antigen presenting cells. This study evaluated the antitumor effect of CIK cells which were non-virally transfected with IL-2 and co-cultured with pulsed and unpulsed DC. Human CIK cells generated from peripheral blood were transfected in vitro with plasmid encoding for the human IL-2. Transfection involved a combination of electrical parameters and a specific solution to deliver plasmid directly to the cell nucleus by .

• Báo cáo khoa học
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học