TAILIEUCHUNG - Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma | Genetic Vaccines and Therapy BioMed Central Research Open Access A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma Ariane Soling 1 Christian TheiE1 Stephanie Jungmichel1 and Nikolai G Rainov1 2 Address 1Molecular Neurooncology Laboratory Dept. Neurosurgery Martin-Luther-University Halle-Wittenberg 06097 Halle Germany and 2Dept. Neurological Science University of Liverpool Liverpool 9L 7LJ United Kingdom Email Ariane Soling - Christian TheiE - christian_theiss@ Stephanie Jungmichel - stephanie_jungmichel@ Nikolai G Rainov - rainov@ Corresponding author Published 04 August 2004 Received 05 April 2004 Accepted 04 August 2004 Genetic Vaccines and Therapy 2004 2 7 doi 1479-0556-2-7 This article is available from http content 2 1 7 2004 Soling et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Suicide gene therapy employing the prodrug activating system Herpes simplex virus type I thymidine kinase HSV-TK ganciclovir GCV has proven to be effective in killing experimental brain tumors. In contrast glioma patients treated with HSV-TK GCV did not show significant treatment benefit most likely due to insufficient transgene delivery to tumor cells. Therefore this study aimed at developing a strategy for real-time noninvasive in vivo monitoring of the activity of a therapeutic gene in brain tumor cells. Methods The HSV-TK gene was fused to the firefly luciferase Luc gene and the fusion construct HSV-TK-Luc was expressed in U87MG human malignant glioma cells. Nude mice with subcutaneous gliomas stably .

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