TAILIEUCHUNG - Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants

Bovine seminal ribonuclease (BS-RNase), a homodimeric protein displaying selective cytotoxicity towards tumor cells, is isolated as a mixture of two isoforms, a dimeric form in which the chains swap their N-termini, and an unswapped dimer. | IFEBS Journal Structure-cytotoxicity relationships in bovine seminal ribonuclease new insights from heat and chemical denaturation studies on variants Concetta Giancola1 Carmine Ercole1 lolanda Fotticchia1 Roberta Spadaccini2 Elio Pizzo3 Giuseppe D Alessio3 and Delia Picone1 1 Department of Chemistry Paolo Corradini University of Naples Federico II Italy 2 Department of Biologicaland EnvironmentalSciences Universita degli Studi delSannio Benevento Italy 3 Department of Structuraland FunctionalBiology University of Naples Federico II Italy Keywords calorimetric analysis chemical denaturation cytotoxic ribonucleases domain-swapping structure-activity relationships Correspondence D. Picone or C. Giancola Dipartimento di Chimica University di Napoli Federico II Complesso Universitario di Monte Sant Angelo Via Cintia 80126 Napoli Italy Fax 39 081674409 39 081674499 Tel 39 081674406 39 081674266 E-mail Received 29 June 2010 revised 17 September 2010 accepted 25 October 2010 doi Bovine seminal ribonuclease BS-RNase a homodimeric protein displaying selective cytotoxicity towards tumor cells is isolated as a mixture of two isoforms a dimeric form in which the chains swap their N-termini and an unswapped dimer. In the cytosolic reducing environment the dimeric form in which the chains swap their N-termini is converted into a noncovalent dimer termed NCD in which the monomers remain intertwined through their N-terminal ends. The quaternary structure renders the reduced protein resistant to the ribonuclease inhibitor a protein that binds most ribonucleases with very high affinity. On the other hand upon selective reduction the unswapped dimer is converted in two monomers which are readily bound and inactivated by the ribonuclease inhibitor. On the basis of these considerations it has been proposed that the cytotoxic activity of BS-RNase relies on the 3D structure and stability of its NCD .

• Báo cáo khoa học
• Scientific reports
• breast cancer
• medicine
• cytotoxicity
• chemical reaction
• tumor cells
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes