TAILIEUCHUNG - Báo cáo sinh học: " Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene | Genet. Sel. Evol. 40 2008 227-239 INRA EDP Sciences 2008 DOI gse 2007046 Available online at Original article Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene Jian Wu1 Donghai ZHOU2 Changyan DENG3 Yuanzhu Xiong3 Minggang LEI3 Fenge Li3 Siwen JIANG3 Bo ZUO3 Rong ZHENG3 1 Department of Basic Veterinary Medicine College of Veterinary Medicine Huazhong Agriculture University Wuhan 430070 P. R. China 2 Department of Clinical Veterinary Medicine College of Veterinary Medicine Huazhong Agriculture University Wuhan 430070 P. R. China 3 Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agriculture University Wuhan 430070 P. R. China Received 12 February 2007 accepted 21 August 2007 Abstract - Carbonic anhydrase III CA3 is an abundant muscle protein characteristic of adult type-1 slow-twitch muscle fibres. In order to further understand the functions of the porcine CA3 protein in muscle the temporal and spatial distributions of its gene product were analysed and the association between the presence of specific polymorphisms and carcass traits in the pig was also examined. Real-time PCR revealed that the CA3 mRNA expression showed no differences with age in skeletal muscles from Yorkshire pigs at postnatal day-1 month-2 and month-4. We provide the first evidence that CA3 is differentially expressed in the skeletal muscle of Yorkshire and Meishan pig breeds. In addition the whole pig genomic DNA sequence of CA3 was investigated and shown to contain seven exons and six introns. Comparative sequencing of the gene from three pig breeds revealed the existence of microsatellite 87160 in intron 5 and microsatellite 87158 and a novel microsatellite marker that includes a tandem repeat of TC n in intron 4. We also determined the allele number and frequencies of the three loci in .

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