TAILIEUCHUNG - Báo cáo " Cell suspension culture of Zedoary (Curcuma zedoaria Roscoe) "

We report here the protocol for Zedoary (Curcuma zedoaria Roscoe) callus and cell suspension cultures. The MS medium supplemented with 2% sucrose, mg/l 2,4-D and mg/l BA was effective for callus induction from in vitro leaf-base explants of Zedoary. During subcultures, secondary proliferated calli were subsequently produced from initial induced calli on the MS medium with mg/l and mg/l BA. These calli were light yellow in color, compact and friable. | VNU Journal of Science Natural Sciences and Technology 27 2011 64-70 Cell suspension culture of Zedoary Curcuma zedoaria Roscoe Vo Chau Tuan1 2 Vu Duc Hoang1 Nguyen Hoang Loc1 1 Institute of Resources Environment and Biotechnology Hue University Hue Vietnam 2College of Education Da Nang University Da Nang Vietnam Received 09 June 2010 Abstract. We report here the protocol for Zedoary Curcuma zedoaria Roscoe callus and cell suspension cultures. The MS medium supplemented with 2 sucrose mg l 2 4-D and mg l BA was effective for callus induction from in vitro leaf-base explants of Zedoary. During subcultures secondary proliferated calli were subsequently produced from initial induced calli on the MS medium with mg l and mg l BA. These calli were light yellow in color compact and friable. The cell suspension culture for Zedoary was established using 3 g fresh weight inoculum in a batch culture on the MS medium supplemented with 3 sucrose mg l 2 4-D and mg l BA. The highest biomass of g fresh weight g dry weight was obtained after 14 days of culture in 50 ml liquid medium of 250 ml Erlenmeyer flask with shaking speed of 120 rpm. Results from this study might be a well established foundation for further studies on Curcuma zedoaria Roscoe in order to serve as a potential source for secondary metabolites production in large scale. Keywords Callus cell biomass cell suspension Curcuma zedoaria medicinal plant 1. Introduction Zedoary Curcuma zedoaria Roscoe plant a vegetatively propagated species of the Zingiberaceae family is an aromatic herbaceous plant with a rhizome growing mainly in South Asian and South-East Asian countries and China 1 . Zedoary is a valuable medicinal plant the essential oil obtained from rhizome has been reported to have antimicrobial activity and be clinically used in the treatment of cervical cancer the water extract of Zedoary Corresponding author. Tel. 84-54-6505051. E-mail nhloc@. vn demonstrated

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