TAILIEUCHUNG - Báo cáo khoa học: Frontal affinity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans

Dendritic cell-specific intracellular adhesion molecule-3-grabbing noninte-grin (DC-SIGN) is a member of the C-type lectin family selectively expressed on immune-related cells. In the present study, we performed a systematic interaction analysis of DC-SIGN and its related receptors, DC-SIGN-related protein (DC-SIGNR) and liver and lymph node sinusoi-dal endothelial cell C-type lectin (LSECtin) using frontal affinity chroma-tography (FAC). | Frontal affinity chromatography analysis of constructs of DC-SIGN DC-SIGNR and LSECtin extend evidence for affinity to agalactosylated N-glycans Rikio Yabe Hiroaki Tateno and Jun Hirabayashi Research Center for MedicalGlycoscience National institute of Advanced Industrial science and Technology AIST Tsukuba Ibaraki Japan Keywords agalactosylated N-glycan C-type lectin DC-SIGN DC-SIGNR LSECtin Correspondence J. Hirabayashi Research Center for Medical Glycoscience NationalInstitute of Advanced IndustrialScience and Technology AIST Tsukuba Ibaraki 305-8568 Japan Fax 81 29 861 3125 Tel 81 29 861 3124 E-mail jun-hirabayashi@ Received 19 August 2009 revised 22 June 2010 accepted 27 July 2010 doi Dendritic cell-specific intracellular adhesion molecule-3-grabbing noninte-grin DC-SIGN is a member of the C-type lectin family selectively expressed on immune-related cells. In the present study we performed a systematic interaction analysis of DC-SIGN and its related receptors DC-SIGN-related protein DC-SIGNR and liver and lymph node sinusoidal endothelial cell C-type lectin LSECtin using frontal affinity chromatography FAC . Carbohydrate-recognition domains of the lectins expressed as Fc-fusion chimeras were immobilized to Protein A-Sepharose and subjected to quantitative FAC analysis using 157 pyridylaminated gly-cans. Both DC-SIGN-Fc and DC-SIGNR-Fc showed similar specificities for glycans containing terminal mannose and fucose but great difference in affinity under the given experimental conditions. By contrast LSECtin-Fc showed no affinity to these glycans. As a common feature the DC-SIGN-related lectin-Fc chimeras including LSECtin exhibited binding affinity to mono- and or bi-antennary agalactosylated N-glycans. The detailed FAC analysis further implied that the presence of terminal GlcNAc at the N-acetylglucosaminyltransferase I position is a key determinant for the binding of these lectins to agalactosylated N-glycans. By .

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• molecular Biology
• tumor cells
• Antimicrobial
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes