TAILIEUCHUNG - Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe"

Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe | Zou et al. Virology Journal 2010 7 37 http content 7 1 37 VIROLOGY JOURNAL RESEARCH Open Access Detection of anatid herpesvirus 1 gC gene by TaqMan fluorescent quantitative real-time PCR with specific primers and probe Qing ZouH Kunfeng Sun13 Anchun Cheng1 2 Mingshu Wang1 2 Chao Xu1 Dekang Zhu2 Renyong Jia2 Qihui Luo2 Yi Zhou3 Zhengli Chen2 Xiaoyue Chen1 2 3 Abstract Background Anatid herpesvirus 1 AHV-1 is known for the difficulty of monitoring and controlling because it has a long period of asymptomatic carrier state in waterfowls. Furthermore as a significant essential agent for viral attachment release stability and virulence gC UL44 gene and its protein product glycoprotein C may play a key role in the epidemiological screening. The objectives of this study were to rapidly sensitively quantitatively detect gC gene of AHV-1 and provide the underlying basis for further investigating DNA vaccine in infected ducks by TaqMan fluorescent quantitative real-time PCR assay FQ-PCR with plasmid. Results The repeatable and reproducible quantitative assay was established by the standard curve with a wide dynamic range eight logarithmic units of concentration and very good correlation values . This protocol was able to detect as little as X 101 DNA copies per reaction and it was highly specific to AHV-1. The TaqMan FQ-PCR assay successfully detected the gC gene in tissue samples from and AHV-1 attenuated vaccine AHV-1 Cha strain inoculated ducks respectively. Conclusions The assay offers an attractive method for the detection of AHV-1 the investigation of distribution pattern of AHV-1 in vivo and molecular epidemiological screening. Meanwhile this method could expedite related AHV-1 and gC DNA vaccine research. Background Anatid herpesvirus 1 AHV-1 infection alternatively known as duck virus enteritis DVE or duck plague DP is one of the most widespread and devastating diseases of waterfowls in the family .

TÀI LIỆU LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.