TAILIEUCHUNG - Báo cáo y học: " Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus"

Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus | Zhang et al. Virology Journal 2010 7 89 http content 7 1 89 VIROLOGY JOURNAL RESEARCH Open Access Fusion to chicken C3d enhances the immunogenicity of the M2 protein of avian influenza virus Zhenhua Zhang 1 2 Yongqing Li 22 Shufang Xu2 Fuyong Chen 21 Li Zhang2 Beiyu Jiang2 and Xiaoling Chen2 Abstract Background Current vaccines to avian influenzae virus AIV a highly contagious disease of birds need to be constantly updated due to the high level of variation in the target antigens. Therefore a vaccine that could induce broad cross protection against AIV is required. The M2 membrane protein is structurally conserved amongst AIV subtypes but tends in induce a poor immune response whereas C3d has been shown in many species to enhance immunogenicity. In this study we investigated the potential of M2-avian C3d fusion proteins to provide effective immunity. Results We fused chicken complement C3d to sM2 M2 protein with the transmembrane region deleted of AIV and expressed four fusion proteins GST Glutathione S-transferase tagged proteins in pGEX expression vector -C3d-sM2 GST-C3d-L2-sM2 GST-C3d-L1-C3d-sM2 and GST-C3d-L1-C3d-L2-sM2 were used to immunize mice. In addition Specific pathogen free SPF chickens were inoculated with the plasmids pcDNA-sM2 pcDNA-C3d-L1-C3d-L2-sM2 GST-sM2 and GST-C3d-L1-C3d-L2-sM2. The immune response was monitored by an enzyme-linked immunosorbent assay ELISA for sM2 antibody and all the test animals were challenged with A chicken Bei Jing WD9 98 H9N2 virus. Results revealed that the anti-sM2 antibody in mice and chickens vaccinated with these proteins was higher than the nonfused forms of sM2 the GST-C3d-L1-C3d-L2-sM2 groups have conferred the highest 30 and 20 protection ratio in mice and chickens respectively. In addition the pcDNA-C3d-L1-C3d-L2-sM2 also enhances the antibody responses to sM2 compared to pcDNA-sM2 in chickens and acquired protection ratio. Conclusion These results indicated that chicken C3d enhanced the

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