TAILIEUCHUNG - Báo cáo khoa học: Enhancing the protein production levels in Escherichia coli with a strong promoter

In biotechnology, the use of Escherichia colifor recombinant protein pro-duction has a long tradition, although the optimal production conditions for certain proteins are still not evident. The most favorable conditions for protein production vary with the gene product. | BFEBS Journal Enhancing the protein production levels in Escherichia coli with a strong promoter Hanna Tegel Jenny Ottosson and Sophia Hober Schoolof Biotechnology Department of Proteomics Royal institute of Technology AlbaNova University Center Stockholm Sweden Keywords Escherichia coli promoter protein production transcription translation Correspondence S. Hober Schoolof Biotechnology Division of Proteomics Royal institute of Technology AlbaNova University Center 106 91 Stockholm Sweden Fax 46 8 55378481 Tel 46 8 55378330 E-mail Received 2 July 2010 revised 5 December 2010 accepted 10 December 2010 doi In biotechnology the use of Escherichia coli for recombinant protein production has a long tradition although the optimal production conditions for certain proteins are still not evident. The most favorable conditions for protein production vary with the gene product. Temperature and induction conditions represent parameters that affect total protein production as well as the amount of soluble protein. Furthermore the choice of promoter and bacterial strain will have large effects on the production of the target protein. In the present study the effects of three different promoters T7 trc and lacUV5 on E. coli production of target proteins with different characteristics are presented. The total amount of target protein as well as the amount of soluble protein were analyzed demonstrating the benefits of using a strong promoter such as T7. To understand the underlying causes transcription levels have been correlated with the total amount of target protein and protein solubility in vitro has been correlated with the amount of soluble protein that is produced. In addition the effects of two different E. coli strains BL21 DE3 and Rosetta DE3 on the expression pattern were analyzed. It is concluded that the regulation of protein production is a combination of the transcription and translation efficiencies. .

• Báo cáo khoa học
• Scientific reports
• breast cancer
• medicine
• strong promoter
• escherichia coli
• production levels
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes