TAILIEUCHUNG - Chapter 066. Stem Cell Biology (Part 2)

Self-Renewal and Proliferation of Stem Cells Symmetric and Asymmetric Cell Division The most widely accepted stem cell definition is a cell with a unique capacity to produce unaltered daughter cells (self-renewal) and to generate specialized cell types (potency). Self-renewal can be achieved in two ways. Asymmetric cell division produces one daughter cell that is identical to the parental cell and one daughter cell that is different from the parental cell and is a progenitor or differentiated cell. Asymmetric cell division does not increase the number of stem cells. Symmetric cell division produces two identical daughter cells. For stem cells to proliferate. | Chapter 066. Stem Cell Biology Part 2 Self-Renewal and Proliferation of Stem Cells Symmetric and Asymmetric Cell Division The most widely accepted stem cell definition is a cell with a unique capacity to produce unaltered daughter cells self-renewal and to generate specialized cell types potency . Self-renewal can be achieved in two ways. Asymmetric cell division produces one daughter cell that is identical to the parental cell and one daughter cell that is different from the parental cell and is a progenitor or differentiated cell. Asymmetric cell division does not increase the number of stem cells. Symmetric cell division produces two identical daughter cells. For stem cells to proliferate in vitro they must divide symmetrically. Self-renewal alone cannot define stem cells because any established cell line . HeLa cells or NIH3T3 cells proliferate by symmetric cell division. Unlimited Expansion In Vitro Resident stem cells are often quiescent and divide infrequently. However once the stem cells are successfully cultured in vitro they often acquire the capacity to divide continuously and the ability to proliferate beyond the normal limit of passages typical of primary cultured cells sometimes called immortality . These features are primarily seen in ES cells but have also been demonstrated for NS cells MS cells MAPCs maGSCs adult-derived tissue stem cells and USSCs newborn-derived tissue stem cells thereby enhancing the potential of these cells for therapeutic use Table 66-1 . Stability of Genotype and Phenotype The capacity to actively proliferate is associated with the potential accumulation of chromosomal abnormalities and mutations. Mouse ES cells have been extensively used to produce gene-targeted animals and are known to maintain their euploid karyotype and genome integrity. In contrast human ES cells appear to be more susceptible to mutations after long-term culture. Another limitation is the possible formation of tumors after transplanting actively .

• Y học thường thức
• Stem Cell Biology
• bệnh học và điều trị
• bài giảng bệnh học
• tài liệu y khoa
• harrison interal medicine
• Essentials of stem cell biology
• Organ development
• Regulation and ethics
• Introduction to stem cells
• BMC Molecular and Cell Biology
• Mesenchymal stem cell
• Cell surface markers
• Bone marrow mesenchymal stem cell
• Breast adipose stem cell
• Fibroblasts and olfactory
• Genome Biology
• Gene expression
• Cell to cell variation
• Stem cell populations
• Intrinsic characteristics
• Single cell sequencing
• Ebook Chemical
• Functional genomic approaches
• Regenerative medicine
• Embryonic stem cells
• Adult stem cells
• Single cell RNA sequencing
• Primed human pluripotent stem cell
• Embryoid body
• Naïve human pluripotent stem cell
• Journal of Biology
• Cell cycle
• Cell death
• Endoderm differentiation
• Inner cell mass (ICM)
• Stem cells
• Mesenchymal stem cells
• Cell biology
• Molecular biology
• Tissue engineering
• Dental pulp
• Genetic stability
• Osteogenic differentiation
• Cell culture models
• Single cell RNA seq
• Definitive endoderm
• Wave Crest
• Human pluripotent
• Stem cells into progenitor
• Glioma stem cell
• Neural stem cell
• DNA mark changes
• Cell proliferation
• Testes somatic stem cells
• JAK/STAT signaling
• Genetically tractable
• Krüppel like factor 2
• Marrow mesenchymal stem cell
• Therapeutic mechanism
• Streptozotocin induced diabetes
• Extracellular matrix
• Self renewal
• Self renewal capacity
• Multiple stem cell markers
• Alternative splicing
• Histone modification
• Cell fate decision
• Cell cycle machinery
• Embryonic stem cell
• Harrisons Internal Medicine
• Applications of Stem Cell Biology
• Clinical Medicine
• Protein characterization
• Biological mass spectrometry
• Mammary stem cell
• Symmetric division
• Progenitor cells
• Droplet digital PCR
• Cell culture
• Chromosome number
• Multiplex assay
• Single cell RNAseq
• In silico lineage tracing
• Neural stem cells
• Schmidtea mediterranea
• Transgenic labeling