TAILIEUCHUNG - Báo cáo y học: "Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus | Hu et al. Virology Journal 2011 8 41 http content 8 1 41 J VIROLOGY JOURNAL RESEARCH Open Access Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus Qiong Hu1 2 Yanbing Niu 1 Kai Zhang1 Yong Liu1 Xueping Zhou1 Abstract Background An effective method for obtaining resistant transgenic plants is to induce RNA silencing by expressing virus-derived dsRNA in plants and this method has been successfully implemented for the generation of different plant lines resistant to many plant viruses. Results Inverted repeats of the partial Tobacco mosaic virus TMV movement protein MP gene and the partial Cucumber mosaic virus CMV replication protein Rep gene were introduced into the plant expression vector and the recombinant plasmids were transformed into Agrobacterium tumefaciens. Agrobacterium-mediated transformation was carried out and three transgenic tobacco lines MP16-17-3 MP16-17-29 and MP16-17-58 immune to TMV infection and three transgenic tobacco lines Rep15-1-1 Rep15-1-7 and Rep15-1-32 immune to CMV infection were obtained. Virus inoculation assays showed that the resistance of these transgenic plants could inherit and keep stable in T4 progeny. The low temperature 15 0 did not influence the resistance of transgenic plants. There was no significant correlation between the resistance and the copy number of the transgene. CMV infection could not break the resistance to TMV in the transgenic tobacco plants expressing TMV hairpin MP RNA. Conclusions We have demonstrated that transgenic tobacco plants expressed partial TMV movement gene and partial CMV replicase gene in the form of an intermolecular intron-hairpin RNA exhibited complete resistance to TMV or CMV infection. Background The plant disease caused by Tobacco mosaic virus TMV or Cucumber mosaic virus CMV is found worldwide. The two viruses are known to infect more than 150 species of herbaceous dicotyledonous plants including many .

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