TAILIEUCHUNG - HPLC for Pharmaceutical Scientists 2007 (Part 3)

The column is the only device in the high-performance liquid chromatography (HPLC) system which actually separates an injected mixture. Column packing materials are the “media” producing the separation, and properties of this media are of primary importance for successful separations. Several thousands of different columns are commercially available, and when selecting a column for a particular separation the chromatographer should be able to decide whether a packed, capillary, or monolithic column is needed and what the desired characteristics of the base material, bonded phase, and bonding density of selected column is needed. Commercial columns of the same general. | 3 STATIONARY PHASES Yuri Kazakevich and Rosario Lobrutto INTRODUCTION The column is the only device in the high-performance liquid chromatography HPLC system which actually separates an injected mixture. Column packing materials are the media producing the separation and properties of this media are of primary importance for successful separations. Several thousands of different columns are commercially available and when selecting a column for a particular separation the chromatographer should be able to decide whether a packed capillary or monolithic column is needed and what the desired characteristics of the base material bonded phase and bonding density of selected column is needed. Commercial columns of the same general type . C18 could differ widely in their separation power among different suppliers. Basic information regarding the specific column provided by the manufacturer such as surface area carbon and type of bonded phase usually does not allow prediction of the separation or for the proper selection of columns with similar separation patterns. Great varieties of different columns are currently available on the market. Four distinct characteristics could be used for column classification 1. Type monolithic porous nonporous 2. Geometry surface area pore volume pore diameter particle size and shape etc. HPLC for Pharmaceutical Scientists Edited by Yuri Kazakevich and Rosario LoBrutto Copyright 2007 by John Wiley Sons Inc. 75 76 STATIONARY PHASES 3. Surface chemistry type of bonded ligands bonding density etc. 4. Type of base material silica polymeric zirconia etc All these characteristics are interrelated. Variations of porosity which include pore diameter can affect both the adsorbent surface area and the bonding density. The type of base material affects adsorbent surface chemistry. Therefore in our discussion we combine these characteristics in two major classes geometry and surface chemistry. Most geometry-related properties of packing .

• Y học thường thức
• stationary phases
• HPLC
• Pharmaceutical Scientists 2007
• HPLC Theory
• tài liệu chuyên ngành dược
• sách học dược sĩ
• Chromatography A
• Cellulose based chiral stationary phases
• Chiral HPLC
• Hydro organic mobile phases compatible
• Retention behaviour
• Enantioresolution ability
• Tetrahydro ß carboline analogs
• Ion exchanger chiral stationary phases
• Enantioselective separation
• Numerous alkaloids
• Mode dedication
• Regeneration of chiral stationary phases
• Eluent history
• Hindered structural transition
• Hydrophilic interaction liquid chromatography
• Homologous series
• Hold up volume
• Column overall solvent volume
• Cinchona alkaloid based chiral stationary phases
• Fluorinated ß phenylalanine derivatives
• Liquid chromatography
• Thermodynamic characterization
• Macrocyclic glycopeptide based chiral
• Core shell particles
• Chiral separation techniques
• Preparative chiral separations
• Method development
• Glycopeptide chiral stationary phases
• Enantiomeric separations
• Bee pollen
• Enantiomeric separation
• Supercritical fluid chromatography
• Tetrahydroisoquinoline analogues
• Benzazepine analogues
• High performance liquid chromatographic
• Subcritical fluid chromatographic
• Isopulegol analogs
• Prodrug mechanism
• Novel antidiabetic drug
• Superficially porous particles
• Low hydrophobicity
• Monoclonal antibody
• Reversed phase liquid chromatography
• High performance liquid chromatography
• Cinchona alkaloid based weak
• Chiral stationary phases
• Mobile phase
• Column screening
• Gradient screening
• Lignosulphonate lignin
• Mass spectrometry
• Phenolic compounds
• Excess isotherm
• Methanol adsorption
• Surface heterogeneity
• Chiral separation
• Enantiomer elution order
• Polar organic mode
• Neat methanol
• High resolution mass spectrometry
• Quantitative structure retention relationship model
• Random forest regression
• Gradient elution