TAILIEUCHUNG - High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients

The association between specific bacteria and colorectal cancer (CRC) has been proposed. Only a few studies have, however, investigated this relationship directly in colorectal tissue with conflicting results. So, we aimed to quantitative Streptococcus gallolyticus, Fusobacterium spp, Enterococcus faecalis and enterotoxigenic Bacteroides fragilis (ETBF) in formalin-fixed and paraffin-embedded (FFPE) colorectal tissue samples of Iranian CRC patients and healthy controls. | Khodaverdi et al. BMC Cancer 2021 21 1353 https s12885-021-09110-x RESEARCH Open Access High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients Nasibeh Khodaverdi1 Habib Zeighami1 Ahmad Jalilvand2 Fakhri Haghi1 and Negar Hesami3 Abstract Background The association between specific bacteria and colorectal cancer CRC has been proposed. Only a few studies have however investigated this relationship directly in colorectal tissue with conflicting results. So we aimed to quantitate Streptococcus gallolyticus Fusobacterium spp Enterococcus faecalis and enterotoxigenic Bacteroides fragilis ETBF in formalin-fixed and paraffin-embedded FFPE colorectal tissue samples of Iranian CRC patients and healthy controls. Methods A total of 80 FFPE colorectal tissue samples of CRC patients n 40 and healthy controls n 40 were investigated for the presence and copy number of above bacterial species using quantitative PCR. Relative quantifica- tion was determined using ΔΔCT method and expressed as relative fold difference compared to reference gene. Results Relative abundance and copy number of E. faecalis and ETBF were significantly higher in CRC samples com- pared to control group. E. faecalis was more prevalent than ETBF in tumor samples. Frequency of ETBF and E. faecalis in late stages III IV of cancer was significantly higher than early stages I II . We did not detect a significant difference in abundance of S. gallolyticus and Fusobacterium spp between two groups. Conclusion Our study revealed the higher concentration of E. faecalis and ETBF in FFPE samples of CRC patients than controls. However additional investigations on fecal and fresh colorectal cancer tissue samples are required to substantiate this correlation. Keywords Bacteroides fragilis Colorectal cancer Enterococcus faecalis Fusobacterium spp Streptococcus gallolyticus Background including genetic .

• Y khoa - Dược
• BMC Cancer
• Bacteroides fragilis
• Colorectal cancer
• Enterococcus faecalis
• Fusobacterium spp
• Streptococcus gallolyticus
• Cancer patient pathways
• Lung cancer
• Breast cancer
• Prostate cancer
• Ovarian cancer
• Cancer detection
• Ovarian cancer symptoms
• Ovarian cancer signs
• Ovarian cancer tests
• Cancer biomarkers
• Pediatric cancer
• Familial cancer predisposition
• Hereditary cancer syndrome
• Genetic cancer susceptibility
• Advanced cancer
• Biliary tract cancer
• Multimodal intervention
• Pancreatic cancer
• Physical activity
• Breast cancer screening
• Familial breast cancer
• Young breast cancer survivors
• High risk relatives
• Randomized trial
• Childhood cancer
• Childhood cancer survivor
• Thyroid cancer screening
• Secondary cancer
• Cancer screening
• Community awareness
• Cancer education
• Cervical cancer
• Prior cancer
• Cancer survivors
• Cancer registry
• Second cancer
• Surgical oncology
• Cancer imaging
• Radiation oncologists
• Medical oncologists
• Oesophagogastric cancer
• Liver cancer
• Hepatobiliary cancer
• Multidisciplinary rehabilitation
• EGFR mutation
• Family history of cancer
• Inherited susceptibility
• Cancer predisposition genes
• Lung cancer aetiology
• Cancer stem cells
• Oct4 overexpressing lung cancer
• Human lung cancer xenograft model
• Disease free survivals
• Cancer hallmarks
• Cancer genesis
• Defiant disease
• Mutational signature
• Cancer diagnosis
• Cancer genomics
• Molecular medicine
• Whole genome sequencing
• Cancer susceptibility gene
• Breast cancer prognosis
• Next generation sequencing
• Quality of care
• Quality indicators
• Shifting cancer care
• German cancer society
• Colorectal cancer screening
• Stomach cancer screening
• Lung cancer survivor
• Physician recommendation
• Total number of lymph nodes
• Lymph node ratio
• Left colon cancer
• Right colon cancer
• Overall survival
• Cumulative incidence
• Esophageal cancer
• Head and neck cancer
• Metachronous cancer
• Young onset
• Reproductive cancer
• Risk factor
• Early detection of cancer
• Gastric cancer incidence
• Population based specialized registry
• Gastric cancer survival
• Gastric cancer clinical characteristics
• Gastric cancer pathological characteristics
• Lung Cancer Screening
• Racial disparities
• Lung Cancer diagnosis
• Screening adherence
• Second primary colorectal cancer
• Standardized incidence ratio
• Left sided colon cancer
• Right sided colon cancer
• Quality of cancer care
• Population based study