TAILIEUCHUNG - Prevalence of ESBL and MBL producing gram negative isolates from various clinical samples in a tertiary care hospital

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern. The prevalence of extended spectrum–lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. A total of 276 Gram negative isolates were processed. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBL detection was done by Imipenem EDTA combined disc diffusion test and Modified Hodge test. Out of 276 isolates 69 (25%) were ESBL producers, 70 () were MBL producers and 26 () were both ESBL and MBL producers. Highest ESBL production was seen in Escherichia coli (46%) and maximum MBL production was seen in Klebsiella pneumoniae ().The study underlines problem of ESBL and MBL mediated resistance, which has created a therapeutic challenge for the clinicians and microbiologists. Simple disk method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains. | Prevalence of ESBL and MBL producing gram negative isolates from various clinical samples in a tertiary care hospital

• Y khoa - Dược
• Prevalence of ESBL
• MBL producing gram negative isolates
• Various clinical samples
• Tertiary care hospital
• Extended spectrum–lactamases
• Klebsiella pneumoniae
• ESBL producing Klebsiella pneumoniae
• Antimicrobial susceptibility pattern
• ESBL producing clinical isolates
• Appropriate infection control measures
• Pseudomonas aeruginosa
• Combined disc test
• MBL producing Pseudomonas aeruginosa
• Escherichia coli
• Neonatal septicemia
• Blood culture
• ESBL in isolates of Klebsiella Pneumoniae
• Klebsiella spp
• Clavulanic acid
• Multidrug resistance
• In vitro antimicrobial susceptibility pattern
• ESBL producing Escherichia coli
• BMC Pharmacology and Toxicology
• Phenotypic expression
• SBL producing Enterobacteriaceae
• Multidrug resistant bacteria
• Antibiotic abuse
• Extended spectrum β lactamase
• Producing bacteria
• Clinical samples
• Multi drug resistance
• Extended spectrum of β lactamase (ESBL)
• Metallo β lactamase (MBL)
• Tertiary care centre
• Diabetic foot ulcer
• Extended spectrum beta lactamase
• SHV and CTXM type genes
• Intensive care units
• Antimicrobial agents
• Drug resistance
• Susceptibility pattern
• Carbapenemase producing isolates
• ESBL producers
• Resistance pattern
• Extended spectrum beta lactamase producers
• Colony characterization
• Durg isolates
• Urine in tirunelveli medical college hospital
• Extended spectrum beta lactamases producing
• ESBL production