TAILIEUCHUNG - Molecular identification and in silico characterization of coat protein in tomato leaf curl virus associated in tomato from south Gujarat region of India

Tomato leaf curl virus disease (ToLCVD) is one of the major causes for highest economical loss of tomato in India. In this study, Natural incidence of ToLCV disease on tomato was observed at Gujarat, India in the March, 2019. The infected plants revealed systemic viral symptoms in the form of sever leaf curling, leaf crumple with marginal yellowing, stem warped and stunted. Molecular methods were followed to further characterized coat protein (CP) gene of tomato leaf curl virus using specified primers. The purified PCR product (~800 bp) was sequenced and further analysed showed that the virus had sequence identities and closest phylogenetic relationships with various isolates of Tomato leaf curl New Delhi virus, therefore name as Tomato leaf curl virus isolate NAU Gujarat coat protein (AV1) gene and submitted to NCBI (). ToLCV coat-protein sequence was computationally analysed to study evolutionally classification and further modelled to predict structure using swiss model that further validated using Ramachadran plot showing amino acid in most favourable region. The present studied may be useful for development of diseases resistance approach for various crops against begomoviruses to minimize the yield losses. | Molecular identification and in silico characterization of coat protein in tomato leaf curl virus associated in tomato from south Gujarat region of India

• Sinh học
• Tomato leaf curl virus
• Whiteflies and phylogenetic relationship
• Phylogenetic relationship
• Tomato leaf curl virus associated
• Silico characterization
• Non chemical management
• Leaf curl virus
• Control of tomato leaf curl virus
• Non chemical management strategies
• Tomato leaf curl disease
• Different germplasm
• Insecticides against tomato leaf curl
• Virus affecting tomato
• Profenofos treatments
• Bemisia tabaci
• Diversity of tomato leaf curl virus
• Chaperone GroEL protein
• Virus particles
• Detection and identification
• Identification of tomato leaf curl virus
• Molecular technique
• PCR method
• Tomato (Solanum lycopersicum L )
• Tomato leaf curl virus disease incidence
• Severity in different tomato growing areas
• Tomato production
• Major limiting factor
• Molecular characterization
• Vein clearing of the leaves
• DNA A molecule
• Solanum lycopersicum
• Bacterial wilt
• Tomato leaf curl virus diseases
• Single cross hybrids
• Tomato (Solanum lycopersicum)
• Screening for identification
• Resistance sources of tomato against
• Segregating population
• Coccinia grandis
• Mixed infection of papaya ring spot virus
• Papaya ring spot virus
• Tomato leaf curl new delhi virus
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Area under disease progress curve
• Dates of planting
• Logistic and gompertz model
• Prediction equation
• Weather parameters
• BMC Plant Biology
• Tomato yellow leaf curl virus
• Salicylic acid
• Ascorbic acid
• ROS scavenging enzymes
• Systemic acquired resistance
• Recombinant coat protein
• TA cloning
• His tag purification
• Cloning and expression
• Coat protein gene
• Simple sequence repeats
• Tomato (Solanum lycopersicum L
• Imparting disease resistance
• Validation of SSR markers
• Susceptible tomato
• Long non coding RNA
• While circular RNAs
• Early blight
• Principal component analysis
• Multivariate analysis
• Physio morpho metric traits
• Genome Biology
• Plant genome engineering
• Synthetic site specific nucleases
• Viral mediated genome editing
• Virus resistance
• Tobacco rattle virus
• BMC Genomics
• Nicotiana benthamiana
• Cellular environment permissive
• Viral replication
• Virus triggered changes
• Dramatic transcriptional reprogramming
• Asia II 1
• Phloem sap sucking pests
• Cryptic species
• Genetic variability
• Molecular genetics
• Restriction fragment length polymorphism