TAILIEUCHUNG - Isolation and screening of carotenoid producing bacteria

This article includes isolation and screening of carotenoid producing bacteria. Samples were collected from different eco-climatic regions of Maharashtra. From soil, mud, sediment, and water samples total 620 bacterial strains were isolated out of which 105 isolates were pigment producers, many of which exhibited bright orange, orange, yellow orange, lemon yellow and red pigmentation. Out of 105 isolates efficient carotenoid producers were screened after primary, secondary, tertiary, and quaternary screening based on pigmentation, tolerance to pH and NaCl%(w/v), degree of pigmentation, absorption maxima and free radicle scavenging activity respectively. The selection of potential carotenoid producer was done from finally screened isolatesSL2, SL4* , SL4-1, SL10, SL2y, DKMIII, DABW1, DGM, SSD2, SWT3 and SS1 based on free radicle scavenging activity by DPPH (1,1 Diphenyl-2 Picryl Hydrazil) method. Isolate SL2 showed bright orange pigmentation, tolerance to pH (6-12), NaCl (1-6%), λmax at 473 nm and maximum 53% free radicle scavenging activity, so it was selected for further studies as a potential carotenoid pigment producer. | 2020 9 1 941-952 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 9 Number 1 2020 Journal homepage http Original Research Article https Isolation and Screening of Carotenoid Producing Bacteria Deepti D. Dhere1 S. M. Dharmadhikari2 and R. S. Pandhare2 1Deogiri College of Arts Science and Commerce Aurangabad . India 2 Government College of Arts and Science Aurangabad India Affiliated to-Dr. Babasaheb Ambedkar Marathwada University Aurangabad . India Corresponding author ABSTRACT Keywords Screening DPPH Carotenoid producing bacteria Article Info Accepted 15 December 2019 Available Online 20 January 2020 This article includes isolation and screening of carotenoid producing bacteria. Samples were collected from different eco-climatic regions of Maharashtra. From soil mud sediment and water samples total 620 bacterial strains were isolated out of which 105 isolates were pigment producers many of which exhibited bright orange orange yellow orange lemon yellow and red pigmentation. Out of 105 isolates efficient carotenoid producers were screened after primary secondary tertiary and quaternary screening based on pigmentation tolerance to pH and NaCl w v degree of pigmentation absorption maxima and free radicle scavenging activity respectively. The selection of potential carotenoid producer was done from finally screened isolatesSL2 SL4 SL4-1 SL10 SL2y DKMIII DABW1 DGM SSD2 SWT3 and SS1 based on free radicle scavenging activity by DPPH 1 1 Diphenyl-2 Picryl Hydrazil method. Isolate SL2 showed bright orange pigmentation tolerance to pH 6-12 NaCl 1-6 Xmax at 473 nm and maximum 53 free radicle scavenging activity so it was selected for further studies as a potential carotenoid pigment producer. Introduction Natural pigments are always favored by human beings as an additive in food to avoid side effects of the artificial mineral

• Sinh học
• Carotenoid producing bacteria
• Screening of carotenoid producing bacteria
• Potential carotenoid pigment producer
• Pigment producers
• Exhibited bright orange