TAILIEUCHUNG - Analysis of Gonadotropin releasing hormone (GnRH), Leutinizing Hormone (LHb) and follicle stimulating hormone (FSHb) genes in Indian spiny loach (Lepidocephalus thermalis)

Lepidocephalus thermalis forms a potential candidate species for aquaculture, being a native species it also has high market demand with easy domestication procedure. RNA was extracted from the ovarian tissues of Lepidocephalus thermalis by following the TRIZOL reagent method and cDNA was synthesized. The purity of RNA, cDNA and PCR products were checked. The PCR reactions were carried out with gene specific primers of gonadotropin releasing hormone II, gonadotropin releasing hormone III, leutinizing hormone and follicle stimulating hormone genes and the PCR products size of the four genes were 270-bp for gonadotropin releasing hormone II, 250-bp for gonadotropin releasing hormone III, 290-bp for leutinizing hormone and 260-bp for follicle stimulating hormone. The results from present study will serve as a primary data for further research and development in the reproductive genomic aspects of Indian spiny loach (Lepidocephalus thermalis). | Analysis of Gonadotropin releasing hormone (GnRH), Leutinizing Hormone (LHb) and follicle stimulating hormone (FSHb) genes in Indian spiny loach (Lepidocephalus thermalis)

• Sinh học
• Lepidocephalusther malis
• Gonadotropin releasing hormone
• Follicle stimulating hormone
• Leutinizing hormone
• Lepidocephalus thermalis
• Steroidogenic enzyme
• Cross specific steroidogenic enzyme genes expression
• Gonadal development
• Steroidogenic enzyme genes
• Indian spiny loach
• Protein feed
• PERProtein efficiency ratio
• SGRS pecific growth rate