TAILIEUCHUNG - Isolation, cloning and sequencing of Phlacbd gene cluster encoding antibiotic and Phloroglucinol derivative 2, 4-Diacetylphloroglucinol (2, 4- DAPG) from Pseudomonas Fluorescens

Pseudomonas represents as one of the most abundant class of bacterial genus in many antagonistic root-associated communities. Pseudomonas spp. residing in the rhizosphere has the ability to produce antimicrobial metabolites including phloroglucinol derivatives 2,4-DAPG, which is a major class of secondary metabolite protecting plants against different types of phytopathogens. The present study was conducted to isolate 2,4-DAPG producing Pseudomonas fluorescens isolates from Rhizosphere soil samples of different crops. Among a total of 158 isolates screened for the production of 2,4-DAPG through PCR based approach, seven isolates were found to be positive for DAPG. Further, the antibacterial activities of these Pseudomonas isolates were evaluated in vitro against Xanthomonas axonopodis pv punicae and it was found that the isolate AFPF19 exhibited highest antagonistic activity against phytopathogen. AFPF19 isolate was used to clone DAPG synthesizing gene cluster phlACBD into cloning vector pTZ257R/T and confirmed by sequencing. | Isolation, cloning and sequencing of Phlacbd gene cluster encoding antibiotic and Phloroglucinol derivative 2, 4-Diacetylphloroglucinol (2, 4- DAPG) from Pseudomonas Fluorescens

• Y khoa - Dược
• Pseudomonas fluorescens
• Diacetylphlorogluci nol
• Phlacbd gene cluster encoding antibiotic
• Phloroglucinol derivative
• Pseudomonas Fluorescens
• Fluorescent pseudomonads
• Rhizoctonia solani
• Disease severity
• Pseudomonas fluorescens against sheath blight
• Pseudomonas fluorescens against sheath blight in rice
• Agricultural science
• Endomycorrhizae and pseudomonas fluorescens
• Vigna unguiculata L
• Salt stress
• Aspergillus flavus
• Scanning electron microscope
• Ultrastructural studies to establish the mode
• Action of Pseudomonas fluorescens
• Sheath blight
• P fluorescens
• Biological management
• Rice in field condition
• Sheath blight disease management
• Pseudomonas fluorescens isolate
• Pseudomonas sp
• Trichoderma sp
• Alternaria porri
• In vitro
• Pseudomonas fluorescens against Alternaria porri
• Biological control
• Pseudomonas bacteria
• Pseudomonas Fluorescens bacteria
• Root knot disease of tomato
• Meloidogyne javanica
• Luận văn tốt nghiệp
• dòng vi khuẩn Pseudomonas fluorescens
• phòng trừ vi khuẩn Ralstonia solanacearum
• bệnh héo xanh trên cây cà chua
• phòng bệnh cây cà chua
• hướng dẫn phòng bệnh cây cà chua
• luận văn
• công nghệ sinh học
• QUY TRÌNH CHUYỂN GENE
• VI KHUẨN Pseudomonas fluorescens
• Phƣơng pháp điện biến nạp
• Phƣơng pháp Calcium chloride
• Protein modeling
• Biosynthesis of pyrrolnitrin
• Pathogenic fungi
• PrnABCD gene cluster codes
• Vanillate demethylase
• Extracellular lipase
• Enzyme purification
• P nitrophenyl esters
• Bacterial enzymes
• Pseudomonas fluorescens HH
• 4 chlorophenol
• Dichlorophenoxyacetic acid
• Loamy soil
• Addition of nitrogen
• Dual culture
• In vitro and in soil
• Rhizoctonia solani in vitro and in soil
• Mycelial growth
• Antagonistic capability
• Estimation of quality parameters
• Antagonistic properties
• Plant disease management
• Trichoderma spp
• Stem rot
• Sclerotium oryzae
• Indo Burma biodiversity hotspot
• Plant growth promotion
• Pseudomonas fluorescens for management
• Tomato in central Himalayas
• Fusarium wilt disease of tomato
• Central Himalayas
• ITS region
• Phylogenetic analysis
• Tomato rhizosphere
• Trichoderma asperellum
• Molecular characterization
• Azotobacter sp
• Black gram
• Vermicompost and enriched compost
• Vigna mungo
• Meloidogyne incognita
• Farmers field of Assam
• Decrease the gaps
• Trichoderma and pseudomonas fluorescens
• Lens culinaris Medik
• Soil borne disease in lentil
• Sclorotinia sclerotiorum
• Silver nanoparticle
• Phyllosphere associated bacterial strain
• Biogenic synthesis of silver nanoparticles
• Biogenic synthesis
• Screening antimicrobial potential
• Copper nanoparticles
• Antimicrobial ability
• Bacillus subtilis
• Insecticides and weedicides
• Pesticides in vitro
• Laboratory condition
• Poorly compatible with propiconazole
• Macrophomina phaseolina
• Pathogen macrophomina phaseolina
• Devastating pathogen right
• Zinc utilization
• Zinc nanoparticles
• Antibacterial activity
• Xanthomonas spp