TAILIEUCHUNG - Molecular characterization of Brucella Melitensis field isolates by bruce- ladder multiplex PCR

Brucellosis still remains an infectious, highly contagious and re-emerging endemic zoonosis especially in the Mediterranean and Middle-East regions of the world involving many countries including India where it constitutes occupational hazard (Shakerian et al., 2013 and Khamesipour et al., 2014). It also poses a serious threat to livestock economy by causing abortion, loss of offspring, infertility and reduction in milk yield. The prevalence of brucellosis in animal reservoirs is an evidence of its prevalence in human population and control of animal brucellosis is the key to its control in humans. Early diagnosis is essential to minimise the spread of the disease besides public health importance. In the present study molecular characterization of five Brucella melitensis isolates was carried out through Bruce-ladder multiplex PCR and compared with Brucella abortus, Brucella melitensis and Brucella suis vaccine and challenge strains. The amplification profile confirmed the isolates as Brucella melitensis and there was a significant difference among these field isolates with that of reference vaccine strains and the amplicons of all the field isolates were similar to amplicons of reference challenge strain, Brucella melitensis 16M. | Molecular characterization of Brucella Melitensis field isolates by bruce- ladder multiplex PCR

• Sinh học
• Brucella melitensis
• Bruce ladder multiplex PCR
• Amplification profile
• Brucella melitensis 16M
• Reference challenge strain
• RBPT and Brucella AMOS PCR
• Caprine flocks in Karnataka
• Brucella melitensis from ovine
• Molecular confirmation
• Real time PCR
• Conventional PCR
• Molecular characterization
• Brucella melitensis detected
• Aborted sheep
• Small ruminants
• Seroprevalence of brucella melitensis
• Humans in anand region
• Enzyme linked immunosorbent
• Brucella melitensis from abortion storms
• Zoonotic implications
• BMC Veterinary Research
• Human brucellosis
• Brucella abortus
• Vaccination program
• Brucella spp
• Nuclear factor κB
• Streptococcus pneumoniae
• Outer membrane protein
• Fc fragment of IgG
• SDS PAGE
• Challenge infection
• Brucella melitensis recombinant
• Nghiên cứu Y học
• Bài viết về y học
• Nhiễm Brucella melitensis
• Gan lách hạch to
• Viêm thân sống L3
• Tổn thương gan
• BMC Genomics
• Whole genome sequencing
• Single nucleotide polymorphism
• Bacterial zoonotic disease
• Brucellosis diagnosis
• Prophylaxis program
• Brucella infected animals
• Single reactors
• Brucellosis eradication programme
• Standardised serological
• Brucellin skin test
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học