TAILIEUCHUNG - Optimization of carbon and nitrogen source for the production of an antimicrobial biopeptide from bacillus firmicutes against food borne pathogensOptimization of carbon and nitrogen source for the production of an antimicrobial biopeptide from bacillus firmicutes against food borne pathogens

The presence and growth of microorganisms in foods is harmful to human and animal health. The consumption of those foods results in food borne diseases. Thus the major concern is the control of microorganisms to increase the shelf life and prevent harmful microorganisms. Bioactive peptides are known for their ability to inhibit protein-protein interactions due to their small size and specificity. Nature remains the largest source of bioactive peptides since plants, animals, fungi, microbes and their products contain various proteins in them. Currently food preservation by the antimicrobial activity of biopeptides against microorganism growth has been studied. The present study is aimed at optimization of the chemical constituents like carbon and nitrogen sources in the production of the biopeptide from the isolated bacteria identified as Bacillus firmicutes based on 16S rRNA sequencing and sequence analysis and its activity against the isolated food pathogens like , S aureus, Pseudomonas aeruginosa, Shigella sps, Salmonella sps and L. monocytogenes. Media optimization for the isolate was conducted by varying the carbon (Fructose, Sucrose, Glucose, Maltose, Starch) and nitrogen (Urea, Ammonium nitrate, Ammonium sulphate, Ammonium dihydrogen phosphate, Sodium nitrate) sources. The Maximum antimicrobial activity was observed with of glucose media against E. coli. Maltose in the medium showed the least inhibitory activity against all the food borne pathogens. The least activity was seen with of the concentration of the carbon sources. The maximum zone of inhibition appeared at of ammonium nitrate against E. coli by the isolate. The least antimicrobial activity was seen against L. monocytogenes in both the carbon and nitrogen sources used in the media by the isolate. No antimicrobial activity was observed with of the nitrogen source in most of the cases. | Optimization of carbon and nitrogen source for the production of an antimicrobial biopeptide from bacillus firmicutes against food borne pathogensOptimization of carbon and nitrogen source for the production of an antimicrobial biopeptide from bacillus firmicutes against food borne pathogens

• Sinh học
• Bacillus firmicutes
• Biopeptide or Bacterio cin
• Antimicrobial activity
• Food borne pathogens
• Food borne pathogens
• Biotechnology and food sciences
• Bacteriophages target
• Food borne pathogens in food industry
• Food borne illnesses
• Bacterial pathogens
• Cell free supernatant
• Inhibitory activity
• Foodborne pathogens
• Against food borne pathogens
• Lactobacillus strains against food borne pathogens
• Ready to eat food products
• PCR detection
• Eat food products
• Milk products
• Lactic acid bacteria
• Food pathogen
• Antibacterial activity
• Zone of inhibition
• Fermented food
• Pathogenic bacteria
• Well diffusion assay method
• P1 – Low density poly ethylene
• P2 – High density poly ethylene
• P3 – Poly propylene
• P4 – Aluminum foil
• Anti microbial activity
• Seed borne pathogens
• Chickpea and poisoned food technique
• Poisoned food technique
• Chickpea seed isolated
• Botanicals against
• Artificial contamination
• Cooking effect
• Core temperature
• Fast food outlets
• Street food vendors
• Cardiac Arrest Survivors
• Percutaneous Intervention
• Leukemia Virus Related
• Blood Borne Pathogens
• Ultrafine and Fine Aerosol
• Safety