TAILIEUCHUNG - A review on transposons and its utilization as genetic tool

Transposable elements, found in almost all organisms, are short sequences of DNA that have the ability to move from one location to the other locations in the genome. Transposons make up 10% of eukaryotic genomes. They provide a means for genomic change and variation, particularly in response to stress (McClintock’s stress hypothesis).There is no known example of transposon playing a normal role in development. They are called as selfish DNA and can be used as genetic markers, as mutagens for transposon tagging and isolation of gene, as transformation vectors and as cloning vehicle. Transposons are potent forces of genetic change and have a significant aspect in the evolution of many genomes. | 2020 9 2 1874-1884 EXCELLENT PUBLISHERS International Journal of Current Microbiology andAppliedSciences ISSN 2319-7706 Volume 9 Number 2 2020 Journal homepage http Review Article https A Review on Transposons and its Utilization as Genetic Tool Sadia Perween Deepak Kumar and Anand Kumar Department of Plant Breeding and Genetics Bihar Agricultural University Sabour Bhagalpur India Corresponding author ABSTRACT Keywords transposons insertational mutagenesis transposon tagging Article Info Accepted 15 January 2020 Available Online 10 February 2020 Transposable elements found in almost all organisms are short sequences of DNA that have the ability to move from one location to the other locations in the genome. Transposons make up 10 of eukaryotic genomes. They provide a means for genomic change and variation particularly in response to stress McClintock s stress hypothesis .There is no known example of transposon playing a normal role in development. They are called as selfish DNA and can be used as genetic markers as mutagens for transposon tagging and isolation of gene as transformation vectors and as cloning vehicle. Transposons are potent forces of genetic change and have a significant aspect in the evolution of many genomes. DNA transposons can be used as genetic tools to introduce a piece of foreign DNA into any genome. They have been utilized for transgenesis and insertional mutagenesis in diverse organisms as these elements are not generally dependent on host factors to facilitate their mobility. Thus DNA transposons are powerful tools to analyze the regulatory genome study the development of embryo identify genes and pathways involved in disease or pathogenesis of pathogens and helps in gene therapy. Transposons are used for gene cloning since insertion of a transposon into a gene upsets its function which develops a visible mutant phenotype. When the DNA sequence of the .

• Sinh học
• Insertational mutagenesis
• Transposon tagging
• Genetic tool
• Utilization as genetic tool
• Review on transposons