TAILIEUCHUNG - Pilot study on molecular quantitation and sequencing of endometrial cytokines gene expression and their effect on the outcome of in vitro fertilization (IVF) cycle

Human trophoblast invasion and differentiation are essential for successful pregnancy outcome. The molecular mechanisms, however, are poorly understood. Interleukin (IL)-11, a cytokine, regulates endometrial epithelial cell adhesion. Leukemia inhibitory factor (LIF) is one of the key cytokines in the embryo implantation regulation. The present study aimed to assess the levels of LIF, IL-11, and IL-11 a receptor gene expression in the endometrium of women undergoing IVF and correlate their levels with the IVF pregnancy outcome. Also, the study aimed to detect any mutation in these three genes among IVF pregnant and non-pregnant women versus control menstrual blood of fertile women. Endometrial tissue biopsies were taken from 15 women undergoing IVF on the day of oocyte retrieval. The quantitative expression of IL-11, IL11Ra, and LIF genes was assessed by real-time PCR and PCR products were sequenced. Menstrual blood from 10 fertile women was used as control to compare the DNA sequence versus DNA sequence of the studied genes in endometrial biopsies. LH, FSH, and E2 were assessed for enrolled patients by ELISA. Endometrial thickness was also assessed by pelvic ultrasonography. No significant difference was detected between quantitative expression of the three studied genes and pregnancy IVF outcome. | Pilot study on molecular quantitation and sequencing of endometrial cytokines gene expression and their effect on the outcome of in vitro fertilization (IVF) cycle

• Y khoa - Dược
• Interleukin 11
• Interleukin 11 receptor a
• Leukemia inhibitory factor
• DNA sequence
• In vitro fertilization
• Pig oocytes
• Pig follicular oocytes
• Cumulus oocyte complexes
• Porcine fertilization medium
• In vitro maturation media on in vitro fertility
• Porcine oocytes of embryos
• Early development of embryos
• In vitro maturation
• Porcine follicular fluid
• Innovative Clinical
• Laboratory Aspects
• Shevach Friedler
• Health
• Sciences
• medicine
• pharmacolocy
• Predictive factors
• Prediction models
• Worldwide using IVF
• The available prediction models in IVF
• Predictive model
• Live birth
• GnRH antagonist
• Embryo transfer
• In vitro fertilization treatment
• Biochemical pregnancy
• Pregnancy loss
• Spontaneous abortion
• Intracytoplasmic sperm injection
• Metaphase II oocyte
• Polarized light microscope
• Fertilization Centre of Vietnam
• BMC Pediatrics
• Oxidative stress
• Bisphenol A
• Bài viết về y học
• Trưởng thành noãn trong ống nghiệm
• Thụ tinh trong ống nghiệm
• Trẻ sinh sống
• Bệnh nhân có số nang thứ cấp
• BMC Genomics
• Embryo development
• In vivo embryo development
• Pre implantation embryos indicate
• Genome Biology
• Early embryos
• Chromatin 3D architecture
• Preimplantation embryos derived
• Zygotic genome activation
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• công trình y học
• nghiên cứu y học
• kiến thức y học
• phụ sản
• triệu chứng bệnh phụ sản
• bệnh phụ sản
• tài liệu về phụ sản giáo án bệnh phụ sản
• medical books
• pharmacology
• epidemiologic
• Obstetrics
• anatomy
• Preventing Morbidity
• Multidisciplinary Care
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• Analysis of Differential
• Genes of Uyghur
• Women with Endometriosis
• Xinjiang
• Primary Afferent
• Nociceptors and Visceral
• Tạp chí khoa học
• Tạp chí y dược
• Y dược quân sự
• Pregnancy rate
• Live birth rate
• Low oxygen concentration
• Melatonin diminishes
• Sperm cells
• Oxidative damage
• Assisted reproduction technology
• Sperm manipulation
• Low oxygen concentration in IVF
• Embryo culture
• Predominantly blastocyst