TAILIEUCHUNG - Inflammatory breast cancer: Activation of the aryl hydrocarbon receptor and its target CYP1B1 correlates closely with Wnt5a/b-b-catenin signalling, the stem cell phenotype and disease progression

The aim of the present study was to evaluate the expression levels of the aryl hydrocarbon receptor (AHR) and its target gene CYP1B1 and to correlate their expression with Wnt5a/b-b-catenin, the CD44+ /CD24(/low) cancer stem cell (CSC) subset and factors associated with poor prognosis in inflammatory breast cancer (IBC) and non-IBC patients. The methods of analysis used were quantitative real-time PCR, western blotting, immunohistochemistry and flow cytometry. Compared to non-IBC tissues, IBC tissues exhibited the overexpression of AHR and its target gene/protein CYP1B1. AHR and CYP1B1 mRNA levels were associated with the poor clinical prognosis markers tumour grade, lymphovascular invasion, cell proliferation and lymph node metastasis. Furthermore, AHR expression correlated with the expression of Wnt5a/b and b-catenin signalling molecules, and Wnt5a mRNA expression was downregulated in the SUM149 human IBC cell line and the MDA-MB-231 non-IBC cell line upon inhibition of AHR. AHR gene knockout (CRISPRCas9) inhibits CYP1B1 and Wnt5a expression in the IBC cell line. The CD44+ /CD24(/low) subset was significantly correlated with the expression of AHR, CYP1B1. | Inflammatory breast cancer: Activation of the aryl hydrocarbon receptor and its target CYP1B1 correlates closely with Wnt5a/b-b-catenin signalling, the stem cell phenotype and disease progression

• Sinh học
• Inflammatory breast cancer
• Aryl hydrocarbon receptor
• Aryl hydrocarbon receptor
• Target CYP1B1 correlates closely
• Stem cell phenotype
• BMC Cancer
• Case comparison study
• Family history of breast cancer
• Breast cancer risk factors
• Multiplex IBC families
• Breast cancer
• Non steroidal anti inflammatory drug
• Hormone receptor positive breast cancer
• HER2 positive breast cancer
• Invasive breast cancer
• Tumor microenvironment
• Aurora A kinase
• Breast cancer specific survival
• Survival rate
• Survival analysis
• Pelvic inflammatory disease
• Uterine cancer
• Ovarian cancer
• Retrospective cohort study
• Indole 3 carbinol
• Canine inflammatory mammary cancer
• Steroid hormones
• Mice model
• Vasculogenic mimicry
• Electron microscopy
• Comparative oncology
• Molecular subtypes
• Overall survival
• Event free survival
• Antiinflammatory cytokines
• Chemotherapy treatment
• Interferon γ
• Inflammatory cytokines
• Nonsteroidal anti inflammatory drugs
• Family history
• Risk factor heterogeneity
• Triple negative breast cancer
• Phospho aspirin
• Non steroidal anti inflammatory drugs
• Epidermal growth factor receptor
• Metabolic reprogramming
• Hyper glycolytic phenotype
• Inflammatory breast neoplasms
• Estrogen receptors
• Gene expression
• Hormone receptor
• Inflammatory mediators induced
• Healthy tissues
• Cancer cell migration
• Metastasis formation
• Cancer stem cells
• Activation of cyclooxygenase
• Nuclear factor κB
• Anti inflammatory drugs
• Tumor generation
• Anti tumor therapeutic
• Viral infection
• IBC is characterized
• Specific morphological
• IBC among young women
• Granulocyte Colony stimulating factor
• Febrile neutropenia
• Wnt/β catenin signaling
• Anti inflammatory
• Antioxidant activities
• Including anti allergic
• Anti viral
• Anti oxidation
• Oestrogen receptor
• Treatment resistance
• Inflammatory cytokine interleukin 6
• Skeletal muscle index
• Systemic inflammation index
• Ferroportin 1
• Stromal inflammatory cells
• Tissue microenvironment
• Rheumatic diseases
• Reactive oxygen species
• Lung cancer