TAILIEUCHUNG - Phylogenetic relationships of Elymus L. and related genera (Poaceae) based on the nuclear ribosomal internal transcribed spacer sequences

To help in the clarification of the taxonomic status of Elymus L. and related genera Agropyron Gaertn., Leymus Hochst., Hordelymus (Jess.) Harz, and Brachypodium ., the sequence diversity in the internal transcribed spacer (ITS) region of nuclear ribosomal DNA was studied. | Research Article Turk J Bot 34 (2010) 467-478 © TÜBİTAK doi: Phylogenetic relationships of Elymus L. and related genera (Poaceae) based on the nuclear ribosomal internal transcribed spacer sequences Ayten DİZKIRICI, Zeki KAYA, Evren CABİ, Musa DOĞAN Department of Biological Sciences, Middle East Technical University, 06531 Ankara - TURKEY Received: Accepted: Abstract: To help in the clarification of the taxonomic status of Elymus L. and related genera Agropyron Gaertn., Leymus Hochst., Hordelymus (Jess.) Harz, and Brachypodium ., the sequence diversity in the internal transcribed spacer (ITS) region of nuclear ribosomal DNA was studied. ITS data of 64 Triticeae accessions including 40 Elymus, 12 Agropyron, 7 Leymus, and 4 Hordelymus, and 1 Brachypodium as an out-group were analysed. The molecular diversity statistics indicated that the most diverse genus is Elymus among the studied genera. The constructed phylogenetic tree by the maximum parsimony method revealed that one specimen of Elymus, E. pycnanthus (Godr.) Melderis, clustered with species of Agropyron. Molecular diversity statistics also indicated that E. pycnanthus is distantly related to other species of Elymus, but is closer to Agropyron species. The finding of strong affinity of Elymus to the species of Leymus and Agropyron supports the view that the taxonomy of Elymus is further complicated by the role of hybridisation among different ancestral genera. Key words: Elymus, molecular diversity, phylogeny, ITS, Triticeae Elymus L. ve akraba cinslerin (Poaceae) nüklear ribozomal iç transkripsiyonu aralayıcı dizisi kullanılarak filogenetik akrabalıkları Özet: Elymus L. ve bu cinse akraba Agropyron Gaertn., Leymus Hochst., Hordelymus (Jess.) Harz ve Brachypodium . cinslerinin taksonomik statülerini açıklamaya yardımcı olmak için nüklear ribozomal DNA bölgesinde bulunan iç transkripsiyonu aralayıcı (ITS) bölgesinin dizi çeşitliliği çalışılmıştır. 40 .

• Nông nghiệp
• Elymus L
• Molecular diversity
• Phylogenetic relationships
• Genera Poaceae
• Internal transcribed spacer
• Nuclear ribosomal DNA
• Dasypyrum villosum
• Elytrigia repens
• Elymus caninus
• Acid phosphatase
• Isoenzyme variation
• Genetic affinities
• BMC Plant Biology
• Elymus s l
• St genome
• Chloroplast gene
• Molecular evolution
• Plant Biology
• Genomic survey sequencing
• Elymus sibiricus L
• Marker development
• Single locus SSR
• Genetic diversity study
• Plastid DNA
• ITS ribosomal DNA
• Molecular phylogeny
• Lilium section Liriotypus
• Balkan peninsulas
• Systematic biology
• Biological macromolecules
• Nuclear genome
• Mitochondrial genome
• Chloroplast genome
• Journal of Botany
• Molecular analyses
• Stalked puffballs
• Fungal DNA barcode
• Scanning electron microscopes
• BMC Genomics
• Satellite DNA
• Fluorescent in situ hybridization
• Natural hybrid
• Primula × uzungolensis
• Molecular characteristics
• Chloroplast DNA
• Genus Lophanthus Adans
• Species Lophanthus turcicus Dirmenci
• Hyssopus lophanthus L
• Flora of China
• DNA sequences
• Morphological characterizations
• Nuclear ribosomal
• Phylogenetic analyses
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Chloroplast capture
• Nuclear ribosomal DNA regions
• Molecular phylogenetic
• Geographic evidence
• Calyx morphology