TAILIEUCHUNG - A novel phytoene synthase paralog from halophytic Salicornia confers salinity tolerance in plants
Salicornia seems to be a suitable euhalophyte for the study of salt stress resilience mechanism and exploitation of salinitytolerant genes for crop yield improvement. In this study, we cloned a novel SePSY1 gene from an extremely salt-tolerant glycophyte, Salicornia europaea. | Turkish Journal of Botany Research Article Turk J Bot (2017) 41: 117-126 © TÜBİTAK doi: A novel phytoene synthase paralog from halophytic Salicornia confers salinity tolerance in plants 1,2 1,2,3 1,2, Nazia REHMAN , Muhammad Ramzan KHAN *, Ghulam Muhammad ALI * PARC Institute for Advanced Studies in Agriculture, National Agricultural Research Centre, Islamabad, Pakistan 2 National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Centre (NARC), Islamabad, Pakistan 3 Present Address: National Centre for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan 1 Received: Accepted/Published Online: Final Version: Abstract: Salicornia seems to be a suitable euhalophyte for the study of salt stress resilience mechanism and exploitation of salinitytolerant genes for crop yield improvement. In this study, we cloned a novel SePSY1 gene from an extremely salt-tolerant glycophyte, Salicornia europaea. The sequence analysis revealed that the newly isolated gene is a paralog of PSY, suggesting a gene duplication event in Salicornia. The newly isolated gene encodes a protein of 419 amino acids. The C-terminus containing a Trans-IPPS-H domain with catalytic and binding regions exhibits considerable conservation in various species, while the N-terminus remained divergent. The structural analysis suggested a catalytic role of the C-terminus in the first step of carotenoid biosynthesis, whereas N-terminus is involved in the transport and localization of protein. Gene expression using real-time RT-PCR revealed enhanced transcript signals for salt-treated plants. Transgenic Arabidopsis plants overexpressing SePSY1 could withstand 200 mM salt stress in comparison with the wild-type counterpart. Thus, SePSY1 confers salinity stress tolerance in the heterologous system. Strength of gene expression is positively correlated with severity of phenotypes.
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