TAILIEUCHUNG - Comparative studies on the competence of axillary shoot regeneration on unsliced and longitudinally sliced cotyledon nodes of Vigna unguiculata

Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. | M. AASIM, S. F. ÖZCAN, K. M. KHAWAR, S. ÖZCAN Research Article Turk J Bot 36 (2012) 281-287 © TÜBİTAK doi: Comparative studies on the competence of axillary shoot regeneration on unsliced and longitudinally sliced cotyledon nodes of Vigna unguiculata Muhammad AASIM1,*, Sancar Fatih ÖZCAN2, Khalid Mahmood KHAWAR2, Sebahattin ÖZCAN2 1 Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Yunus Emre Campus, 70200 Karaman - TURKEY 2 Department of Field Crops, Faculty of Agriculture, Ankara University, 06110 Dışkapı, Ankara - TURKEY Received: Accepted: Abstract: Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing , , , and μM benzylaminopurine (BA) supplemented with mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. The shoot regeneration frequency (%) of longitudinally sliced cotyledon node explants was 2-fold to 3-fold higher than that of unsliced cotyledon node explants. A maximum number of shoots per longitudinally sliced cotyledon node explant was recorded on MS medium containing μM BA. Each increase in the BA concentration of the culture medium proportionally decreased the mean shoot length on both explants. Maximum mean shoot lengths of cm on unsliced cotyledon nodes and cm on longitudinally sliced cotyledon node explant were recorded on MS medium containing μM BA. Regenerated shoots were rooted on MS rooting medium containing .

• Nông nghiệp
• Vigna unguiculata
• Agricultural constraints
• Biotechnological methods
• Indole 3 butyric acid
• In vitro regenerated plants
• Cowpea [Vigna unguiculata (L ) Walp ]
• F2 generation of cowpea
• Genetic advance studies
• Genotypic variances
• Estimates of phenotypic
• Plant genetic engineering
• Cowpea Vigna unguiculata L
• Herbicide tolerance
• N acetyltransferase
• Herbicide tolerance gene
• Genetic variability
• Path coefficient analysis
• Yield and its attributing traits
• Cowpea [Vigna unguiculata (L
• ) Walp]
• Randomized block design
• Path co efficient analysis
• Correlation and path analysis
• Path analysis
• Yield and yield components
• Yard long bean
• Long podded cowpea
• Snake bean
• Vigna unguiculata sub spp Sesquipedalis
• Aphis craccivora
• Seasonal incidence of aphid
• Colletotrichum lindemuthianum
• Bio agents
• Cowpea (Vigna unguiculata)
• Eco friendly management
• Anthracnose disease
• Fertilizer management
• Land configuration
• Quality content
• Vigna unguiculata L
• Yield and yield attributes
• Cowpea (Vigna unguiculata L
• Walp)
• Trait association and path analysis
• Genetics and plant breeding
• Combining ability
• Line X tester
• Line X tester analysis
• Combining ability in cowpea
• Generation mean analysis
• Cowpea and Micronutrients
• Quantitative traits in cowpea
• Six parameters genetic model
• Helminthosporium Vignicola
• Target leaf spot
• Target leaf spot disease
• Foliar fungal pathogen experienced
• D2 statistics
• Genetic diversity
• Mahalanobis D2 analysis
• ) Walp
• Genetic divergence studies
• Hot arid climate
• Accession x environment interaction
• Crossover and noncrossover interactions
• Regression analysis
• Cowpea [Vigna unguiculata (L )]
• F2 segregants
• Dual purpose
• Cowpea [Vigna unguiculata (L )Walp ]
• Dual purpose traits in F2 populations
• Fertilizer recommendation
• Yield target
• Nutrient requirement
• Soil test crop response studies
• Dual purpose cowpea
• Heritability and variability
• Cowpea (Vigna unguiculata (L ) Walp )
• Grain yield related characters in F2 populations
• Vegetable cowpea
• Plant growth
• Vegetable cowpea [Vigna unguiculata (L ) Walp]
• Respect to plant growth
• Fruiting behavior
• Standard heterosis
• Green pod yield per plant
• Green pod yield and attributing characters
• Attributing characters in cowpea
• SSR Marker
• DNA fingerprinting
• Tamil Nadu [Vigna unguiculata (L ) Walp ]
• Cowpea varieties of Tamil Nadu
• SSR marker based DNA fingerprinting
• Generative growth
• Sink capacity
• Grain yield per plant
• Four cowpea (Vigna unguiculata L Walp) genotypes
• Yield and yield attributing characters
• F2 population
• F2 generations
• Cowpea (Vigna unguiculata (L
• ) Walp)
• Moisture stress
• Cowpea (Vigna unguiculata L walp )
• Different stages of growth
• Leaf relative water content
• F4 Segregants
• Bruchid (Callosobruchus maculates F )
• Resistance studies in F4 segregants
• Bruchid infestation
• Segregants F4 191
• Seed yield and it’s contributing traits
• Genetic architecture
• Future breeding programs