TAILIEUCHUNG - Electrochemical dna sensor for herpes virus detection

The results showed that the DNA sensor can determine the DNA target of HSV-1 in the sample at the concentration as low as nM. The response time of the DNA sensor is less than a minute. Temperature effect was also investigated in this work. | Journal of Chemistry, Vol. 46 (1), P. 127 - 132, 2008 Electrochemical DNA sensor for Herpes virus detection Received 23 July 2007 Phuong Dinh Tam1,2, Mai Anh Tuan1,2, Tran Quang Huy4, Nguyen Duc Chien2,3 1 Hanoi Advanced School of Science and Technology 2 International Training Institute for Materials Science (ITIMS) 3 Institute of Engineering Physics, Hanoi University of Technology 4 National Institute of Hygiene and Epidemiology summary This paper reports the development of electrochemical DNA sensor for biomedical application. The micro-sensor was fabricated at Hanoi University of Technology clean room by microelectronic technology. Two pairs of electrodes of which optimized dimension is 70 μm x 30 μm (the width of electrode x inter-distance between fingers), were created on a chip for differential measurements. The DNA of herpes simplex virus 1 (HSV-1) with the DNA probe sequence is 5 AT CAC CGA CCC GGA GAG GGA C 3 was immobilized onto the surface of the sensors by means of 3-aminopropyltriethoxysilance (APTS). The results showed that the DNA sensor can determine the DNA target of HSV-1 in the sample at the concentration as low as nM. The response time of the DNA sensor is less than a minute. Temperature effect was also investigated in this work. Keywords: DNA sensor, electrochemical detection, APTS, virus, hybridization. I - Introduction In recent years, many works have been devoted to DNA sensor development thanks to simplicity, specificity, exceptional sensitivity and selectivity for the detection of specific genes [1 - 3]. This technology has opened a perspective to overcome most of disadvantages of the conventional methods for applications in food, agriculture, environment, clinic and drug etc [3 - 12]. The detection of specific sequences in clinical samples collected from patients diseases is very important in DNA prediagnostics which allows us a time long enough to find out the solution for that [8 - 12]. With the ability of amplification from

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