TAILIEUCHUNG - Molecular cloning and functional characterization of a Na+/H+ antiporter gene from halophyte Spartina anglica
Na+/H+ antiporters have been suggested to play important roles in salt tolerance in plants. English cordgrass (Spartina anglica) is a gramineous halophyte with very strong salt tolerance. It possesses salt glands in its stems and leaves, through which excess salt can be excreted. | T. LAN, Y. DUAN, B. WANG, Y. ZHOU, W. WU Research Article Turk J Agric For 35 (2011) 535-543 © TÜBİTAK doi: Molecular cloning and functional characterization of a Na+/H+ antiporter gene from halophyte Spartina anglica Tao LAN1,2,3, Yuanlin DUAN1,2,3, Bin WANG1,4, Yuanchang ZHOU1,2,3, Weiren WU1,2,4,* 1 Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, Fuzhou, Fujian, 350002 - P. R. CHINA 2 Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding, Fuzhou, Fujian, 350002 - P. R. CHINA 3 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002 - P. R. CHINA 4 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002 - P. R. CHINA Received: Abstract: Na+/H+ antiporters have been suggested to play important roles in salt tolerance in plants. English cordgrass (Spartina anglica) is a gramineous halophyte with very strong salt tolerance. It possesses salt glands in its stems and leaves, through which excess salt can be excreted. In this study, a vacuolar Na+/H+ antiporter homologous gene (temporarily named SaNHX1) was isolated from Spartina anglica by RT-PCR and rapid amplification of cDNA ends (RACE). Southern blot analysis suggested that there might be 2 or 3 copies of the vacuolar Na+/H+ antiporter genes in the English cordgrass genome. Northern blot analysis showed that the expression of vacuolar Na+/H+ antiporter genes in English cordgrass is induced by salt stress. Overexpression of SaNHX1 driven by constitutive promoter Ubi-1 in rice significantly enhanced the salt tolerance of transgenic plants, validating the function of SaNHX1 and suggesting its value for the genetic improvement of salt tolerance in plants. Key words: Halophyte, salt tolerance, SaNHX1, Spartina anglica, transgenic rice Introduction There are approximately 830 million hectares of saline-alkali soil in the world, .
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