TAILIEUCHUNG - Occurrence of Botryosphaeriaceae species associated with grapevine dieback in Algeria

Grapevine dieback is an increasing problem in grape vineyards in Algeria. We conducted field surveys each spring between 2006 and 2012 to determine the fungal pathogens associated with grapevine decline in various grape-growing regions. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 865-876 © TÜBİTAK doi: Occurrence of Botryosphaeriaceae species associated with grapevine dieback in Algeria 1,2 2 1 3 Faiza AMMAD , Messaoud BENCHABANE , Mohamed TOUMI , Nesrine BELKACEM , 3 4 5 6,7, Amel GUESMI , Cherif AMEUR , Pascal LECOMTE , Othmane MERAH * 1 Department of Biology, Superior Normal School Kouba, Vieux-Kouba, Algiers, Algeria 2 Department of Biotechnology, Faculty of Sciences of Nature and Life, University of Blida, Algeria 3 Laboratory of Microorganisms and Active Biomolecules, Department of Biology, Faculty of Sciences of Tunis, University Campus, Tunis, Tunisia 4 LR11-ES31 Biotechnology and Valorization of Bio-Geo Resources, Higher Institute for Biotechnology, University of Manouba, Sidi Thabet Biotechpole, Sidi Thabet, Ariana, Tunisia 5 INRA, ISVV, UMR 1065 Plant Health and Agroecology Vineyard, Villenave d’Ornon, France 6 Agro-Industrial Chemistry Laboratory, University of Toulouse, INP-ENSIACET, Toulouse, France 7 INRA, UMR 1010 CAI, Toulouse, France Received: Accepted: Published Online: Printed: Abstract: Grapevine dieback is an increasing problem in grape vineyards in Algeria. We conducted field surveys each spring between 2006 and 2012 to determine the fungal pathogens associated with grapevine decline in various grape-growing regions. We isolated and identified 3 species of Botryosphaeriaceae (Botryosphaeria dothidea, Diplodia seriata, and Neofusicoccum parvum) from infected wood using a combination of morphological and cultural characters, as well as phylogenetic analyses of ribosomal DNA internal transcribed spacer (ITS) regions and fruiting body. We identified 20 isolates of Diplodia seriata, 13 isolates of Botryosphaeria dothidea, and 7 isolates of Neofusicoccum parvum. Inoculation of grapevine plantlets with the 3 .

• Nông nghiệp
• Botryosphaeriaceae species
• Grapevine trunk diseases
• Pathogenicity test
• Phylogenetic analysis
• Grape growing regions