TAILIEUCHUNG - Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies
The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes however amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 288-299 © TÜBİTAK doi: Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies 1,2, 1 3 1 Tayyaba SHAHEEN *, Yusuf ZAFAR , James M. STEWART , Mehboob-ur RAHMAN Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan 2 Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Pakistan 3 Department of Crop, Soil, and Environmental Sciences, Division of Agriculture, University of Arkansas, Fayetteville, Arkansas 72701, USA 1 Received: Accepted: Published Online: Printed: Abstract: Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500–800 bp long were analyzed for genomic simple sequence repeats (gSSRs). From 39 segments, 23 short gSSRs were identified; on average they occurred every kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum (AD). The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored. Key words: Gossypium, phylogenetic assessment, RAPD, short gSSRs 1. Introduction DNA markers offer an expedient way to study polymorphisms and their use in crop improvement. PCR-based markers are either sequence-specific or random (Gupta et al. 1999). Sequence specific markers are preferred over random markers because of the unreliability of random markers (Rahman et al. 2002; Guo et
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