TAILIEUCHUNG - Molecular cloning of BnZEP and its expression in petals of different colors in Brassica napus L.

Carotenoids are a class of pigment elements present in photosynthetic organisms. T he transcripts of specific carotenoid biosynthetic genes are probably important for the formation of different colors of flower petals. Zeaxanthin epoxidase (ZEP) enzyme activity is important for the high content of carotenoids in petals. In the present study, the zeaxanthin epoxidase gene in Brassica napus L. (BnZEP) was cloned and bioinformatically analyzed | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2015) 39: 377-386 © TÜBİTAK doi: Molecular cloning of BnZEP and its expression in petals of different colors in Brassica napus L. Jun JIANG*, Lushen TIAN*, Shixing GUO, Qingqing YU, Dezhi ZENG, Yingze NIU** Rapeseed Research Center, Sichuan Agricultural University, Wenjiang, Sichuan, . China Received: Accepted: Published Online: Printed: Abstract: Carotenoids are a class of pigment elements present in photosynthetic organisms. T he transcripts of specific carotenoid biosynthetic genes are probably important for the formation of different colors of flower petals. Zeaxanthin epoxidase (ZEP) enzyme activity is important for the high content of carotenoids in petals. In the present study, the zeaxanthin epoxidase gene in Brassica napus L. (BnZEP) was cloned and bioinformatically analyzed. The expression levels of the BnZEP gene in different plant organs and petals of different colors were investigated and compared in B. napus. The results showed that the full-length cDNA sequence of BnZEP was 2228 bp, containing an open reading frame of 2010 bp and encoding a putative protein of 669 amino acids, with a molecular weight of kDa and an isoelectric point (pI) of . The full-length genomic DNA sequence of BnZEP was 3268 bp, containing 16 exons and 15 introns, the same as in Arabidopsis thaliana (AtZEP). The predicted amino acid sequence of BnZEP revealed a close phylogenetic relationship among the cruciferous species, including Arabidopsis thaliana. The expression levels of BnZEP were remarkably different in different organs and in petals of different colors. Obviously higher expression levels of BnZEP were found in flower petals than in leaves and buds. Yellow petals had markedly higher expression levels of BnZEP than white petals. The results showed that the expression .

• Nông nghiệp
• Brassica napus L
• Flower color
• Gene cloning
• Real time quantitative PCR
• Zeaxanthin epoxidase
• Yield attributes
• Yield of Gobhi sarson
• Influence of sulphur application
• Secondary branches per plant
• BMC Plant Biology
• Thermo sensitive genic male sterility
• Microscopic observation
• Multiplex PCR analysis
• Statistical models
• Nested association mapping
• Double haploid NAM
• Backcross NAM
• Computer simulations
• Linkage mapping
• Regional association mapping
• Seed weight
• Silique length
• Fine mapping
• Linkage disequilibrium
• BMC Genomics
• Tribenuron methyl
• Seed germination
• Response mechanism
• Sulfonylurea acetolactate synthase
• Agricultural science
• Varieties of canola plant
• Newly reclaimed desert to plant growth promoting rhizobacteria
• Mineral nitrogen fertilizer
• Nitrogen fertilization
• Plant Biology
• High affinity nitrate transporter
• Various stresses
• Nitrate absorption
• Plant species
• Antioxidant enzyme
• Brassica napus
• Drought stress
• Gene expression
• Physiological traits
• Quantitative trait loci mapping
• Crop production
• Biometrical methods
• Framework map
• Cold stress
• Transcription factor
• Short day
• Genetic diversity
• Correlation coefficients
• Path analysis
• Cluster analysis
• Cuticular wax
• Genome wide association study
• RNA seq
• Single nucleotide polymorphism
• Chlorophyll content
• Near isogenic line
• Abiotic stresses
• Sclerotinia sclerotiorum
• Despite extensive
• Association analysis
• Multi silique
• Transcriptome sequencing
• Whole genome re sequencing
• Flowering time
• Quantitative trait loci
• RNA Seq analysis
• Seed oil content
• Lipid degradation
• Graphene oxide
• Root growth
• Transcript level
• B napus L
• Indole 3 acetic acid
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Boron deficiency
• Boron uptake
• Genotypic differences
• Oilseed rape
• Screening methods
• Arabidopsis thaliana
• Phenylalanine ammonia lyase
• Yellow seed
• Phenylalanine ammonia lyase 2 genes
• Biological yield
• Critical period
• Grain yield
• Oil yield
• Weed interference
• Transplanted gobhisarson
• Organic manures
• Inorganic fertilizers
• Poultry manure
• Nitrogen and yield attributes