TAILIEUCHUNG - Effect of the ethylene inhibitors silver nitrate, silver sulfate, and cobalt chloride on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6
In the present study, the effects of three ethylene inhibitors, silver nitrate (AgNO3), silver sulfate (Ag2SO4), and cobalt chloride (CoCl2), on the morphogenic and biochemical responses in CAB-6P and Gisela 6 cherry rootstocks were investigated. | Turkish Journal of Biology Research Article Turk J Biol (2016) 40: 670-683 © TÜBİTAK doi: Effect of the ethylene inhibitors silver nitrate, silver sulfate, and cobalt chloride on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6 Virginia SARROPOULOU*, Kortessa DIMASSI-THERIOU, Ioannis THERIOS Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece Received: Accepted/Published Online: Final Version: Abstract: In the present study, the effects of three ethylene inhibitors, silver nitrate (AgNO3), silver sulfate (Ag2SO4), and cobalt chloride (CoCl2), on the morphogenic and biochemical responses in CAB-6P and Gisela 6 cherry rootstocks were investigated. In both rootstocks, AgNO3 and Ag2SO4 promoted shoot regeneration while CoCl2 had such an effect only in Gisela 6. The rooting percentage of CAB-6P and Gisela 6 explants was diminished with AgNO3, Ag2SO4, and CoCl2. AgNO3 increased root number (40 µM) and root length (50 µM) in the CAB-6P rootstock while it had an inhibitory effect in Gisela 6. Ag2SO4 and CoCl2 enhanced root length of Gisela 6 explants whereas Ag2SO4 decreased root number and root length of CAB-6P explants. CoCl2 at 10 µM in both rootstocks augmented root number whereas 20 µM CoCl2 increased root length of CAB-6P microshoots. AgNO3, Ag2SO4, and CoCl2 decreased leaf chlorophyll and carbohydrate content in CAB-6P rootstock. On the other hand, in Gisela 6, 10 µM AgNO3 gave elevated chlorophyll levels and 20 µM AgNO3 or 10–50 µM CoCl2 raised leaf carbohydrate content. The three ethylene inhibitors led to elevated leaf proline levels in the CAB-6P rootstock, whereas in Gisela 6 the increase or decrease in leaf proline content was dependent on both type and concentration of ethylene inhibitor. An efficient micropropagation protocol was established
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