TAILIEUCHUNG - Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species
Several species within the genus Lotus are important forage crops, and many are endangered or rare. Despite the high genetic diversity of the genus, identification of Lotus species is problematic because of the limited number of reliable morphological markers. | Turkish Journal of Botany Turk J Bot (2018) 42: 1-14 © TÜBİTAK doi: Research Article Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species 1,2 1, 1 2 1 Erik DUCÁR , Monika REWERS *, Iwona JEDRZEJCZYK , Pavol MÁRTONFI , Elwira SLIWINSKA Laboratory of Molecular Biology and Cytometry, Department of Plant Genetics, Physiology, and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland 2 Department of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Košice, Slovakia 1 Received: Accepted/Published Online: Final Version: Abstract: Several species within the genus Lotus are important forage crops, and many are endangered or rare. Despite the high genetic diversity of the genus, identification of Lotus species is problematic because of the limited number of reliable morphological markers. In search of a quick, inexpensive, and steady method for species identification, genome size and cell cycle/endoreduplication intensity of 14 accessions belonging to eight Lotus species were estimated by flow cytometry. ISSR-PCR was also applied to find sensitive molecular markers for genetic diversity estimation. Genome size estimation revealed that Lotus species possess very small genomes and this characteristic enables the identification of five out of eight species. However, a flow cytometric study of cell ploidy/endopolyploidy in seeds and seedlings enabled us also to distinguish the remaining species. Thus, it is proposed here that combined flow cytometric analyses (the estimation of genome size and cell cycle/endoreduplication pattern) can be applied for screening of Lotus species. Nonetheless, ISSR markers provided a more precise identification of studied accessions, including detection of genetic diversity within a species. Most of the tested primers revealed .
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