TAILIEUCHUNG - Melatonin diminishes oxidative damage in sperm cells, improving assisted reproductive techniques

Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. | Turkish Journal of Biology Turk J Biol (2017) 41: 881-889 © TÜBİTAK doi: Research Article Melatonin diminishes oxidative damage in sperm cells, improving assisted reproductive techniques 1,2 1 1 2 2 Fabián MONLLOR , Javier ESPINO , Ana María MARCHENA , Águeda ORTIZ , Graciela LOZANO , 2 1 1 1, Juan Francisco GARCÍA , José Antonio PARIENTE , Ana Beatriz RODRÍGUEZ , Ignacio BEJARANO * 1 Department of Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain 2 Centre for Human Assisted Reproduction (CERHA), Badajoz, Spain Received: Accepted/Published Online: Final Version: Abstract: Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 allowed the monitoring of acrosome reaction and apoptosis by flow cytometry. Sperm DNA fragmentation and compaction were analyzed through propidium iodide staining. The normozoospermic and oligozoospermic samples that were preincubated with melatonin underwent a significant increase in the ratio of adequate spermatozoa and a reduction of caspase-3 activation. Additionally, preincubation with melatonin enhanced the migration of sperm cells with compacted DNA in oligozoospermic samples (P < ) and prevented DNA fragmentation in normozoospermic samples (P < ). In light of the current results, the .

• Sinh học
• Melatonin diminishes
• Sperm cells
• In vitro fertilization
• Oxidative damage
• Assisted reproduction technology
• Sperm manipulation