TAILIEUCHUNG - Development of transgenic tobacco lines with pyramided insect resistant genes

Insect pests are among the major constraints rendering drastic decreases in crop yield. The expression of stacked insecticidal genes in crops can lead to resistance durability and can delay the development of resistance in target insect pests. | Turkish Journal of Biology Turk J Biol (2018) 42: 174-186 © TÜBİTAK doi: Research Article Development of transgenic tobacco lines with pyramided insect resistant genes 1, 1 1 1 2 1 Allah BAKHSH *, Tolga DİNÇ , Tahira HUSSAIN , Ufuk DEMİREL , Muhammad AASIM , Mehmet Emin ÇALIŞKAN 1 Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, Niğde, Turkey 2 Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, Konya, Turkey Received: Accepted/Published Online: Final Version: Abstract: Insect pests are among the major constraints rendering drastic decreases in crop yield. The expression of stacked insecticidal genes in crops can lead to resistance durability and can delay the development of resistance in target insect pests. The present study was designed to introduce an insect resistance trait in locally cultivated Turkish tobacco cultivars (Basma and Nail) with pyramided insecticidal genes. Agrobacterium strain LBA4404 harboring plasmid pKGH4 with cry1Ac and cry2A genes under the control of 35S promoter was used to infect leaf discs of both cultivars; plasmid also contained uidA within the T-DNA region for earlier screening of putative transformants. The overall transformation efficiency was calculated as and in Basma and Nail, respectively. PCR results confirmed the integration of cry1Ac, cry2A, uidA, and nptII genes in 40 plants of Basma and 16 plants of Nail. ELISA results showed variation in expression of cry1Ac protein among transgenic plants varying from to µg/g of fresh tissue. Bioassay results with potato tuber moth (Phthorimea operculella Zeller) showed significant mortality of the targeted pest on primary transformants. Furthermore, T1 transgenic progeny exhibited the inheritance of T-DNA in Mendelian as well as non-Mendelian .

• Sinh học
• Turkish tobacco
• Genetic transformation
• Protein expression
• Insect resistance
• Crop yield
• Petroleum Products Industry
• Pharmaceutical Industry
• Leather and Leather Goods Industry
• Textile and Clothing Industry
• Tobacco Products Industry
• Refractory Materials Industry
• Salinity tolerance
• Nicotiana tabacum L
• Antioxidative enzymes
• Glutathione reductase
• Proline accumulation
• BMC Pulmonary Medicine
• Smoking related deaths
• Health care professionals
• Physicians’ smoking
• Turkish thoracic society
• Nutrient management
• Crop yield and quality
• Wheat crop
• Efficient nutrient management
• High crop yield
• Quality in wheat crop
• Crop establishment methods
• Grain yield
• Different crop establishment methods
• Yield attributes
• Net income
• Integrated crop management approach
• Following sustainable
• Integrated crop management practices
• Residue on yield
• Yield attributes and economics
• Economics of ricein RW system
• Northern plains of IGP
• Herbicide on growth
• Mustard crop
• Yield of the mustard crop
• Randomised block
• Soil properties
• Crop yield under boron nutrition management
• Paddy crop in Coastal Karnataka
• Nutrient management studies
• Soil available nutrients yield
• Crop geometry
• Yield and yield attributes
• Hybrid mustard as affected
• Crop geometry and varieties
• Their bigger canopy
• Harvest index
• Total grain yield
• Biological yield in K 27 variety
• Maize crop
• Plant growth regulators
• Rice (Oryza sativa L )
• Nutrients on yield
• Yield attributing characters
• Rice crop
• Field experiment on rice crop
• Regression models
• Yield forecasting
• Crop yield forecasting of sorghum
• Crop yield forecasting
• Statistical technique
• Potato (Solanum tuberosum L )
• Non crop mulch
• Rice eco system
• Non crop mulch management
• Varying moisture regimes
• Yield of zero tillage potato
• Rain fed low land rice eco system
• Weed dynamics
• Crop growth parameters and yield
• Herbicides weed management effect
• Crop growth
• Yield in rice
• Growth and yield
• Potato crop
• Plant height
• Yield parameters of potato crop
• Depth of tuber planting on growth
• Time of planting
• Growth and yield parameters
• Planting on growth
• Growing degree days
• Photothermal units
• Crop growth rate
• Yield attributes of quinoa
• Varied crop geometry
• Physico chemical
• Yield attributes and yield
• Rain fed condition
• Eastern part of Uttar Pradesh
• Yield of maize crop
• Integrated nutrient management on yield
• Influence of date
• Foliar application
• Nutrients on crop growth
• Seed yield of soybean
• Seed yield
• Front line demonstration
• Yield gap
• Front line demonstrations on yield
• Economics of mustard crop
• Yield gaps between improved package
• Straw yield
• Lowland farming situation
• Yield of rice
• Summer under lowland farming situation
• Vermicompost Rubbish
• Tomato crop
• Soil health
• Selected vegetable crop
• Vermicompost and vermicompost rubbish
• Growth and yield effects
• Integrated nutrient management
• Nutrient content
• Yield of rice crop
• Rice crop in Inceptisol
• Crop residue management practices
• Growth and yield of wheat
• Triticum aestivum L
• Rice wheat system
• Crop residue management
• Entrapped preceding rice crop herbicide oxadiargyl
• Herbicide oxadiargyl
• Entrapped preceding rice crop
• Yield of succeeding bhendi
• Succeeding bhendi
• CO2 and CERES model
• Wheat crop in eastern India
• Yield of wheat crop
• Climate change impacts on growth
• Imitation of climate change impacts
• Vital role
• Sub surface drainage system
• Soil chemical properties
• Rice crop at head region
• Tungabhadra command area