TAILIEUCHUNG - Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines

Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate. | Turkish Journal of Biology Turk J Biol (2018) 42: 132-143 © TÜBİTAK doi: Research Article Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines 1, 1 2 Sibel ÖZDAŞ *, Talih ÖZDAŞ Department of Bioengineering, Faculty of Engineering and Natural Sciences, Adana Science and Technology University, Adana, Turkey 2 Otolaryngology Clinic, Adana Numune Education and Research Hospital, Adana, Turkey Received: Accepted/Published Online: Final Version: Abstract: Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate. Consequently, there is a need to explore the molecular characteristics of this cancer in order to discover potential therapeutic target molecules. The overexpression of chromosome region maintenance 1 (Crm1), responsible for the transport of different classes of macromolecules from the nuclear membrane to the cytoplasm, in various cancer cells has made it an attractive target molecule in cancer research. It has been reported that transcription factors, which are the target cargo proteins of Crm1, have critical roles in regulating intracellular processes via their expression levels and functions, which in turn are regulated by the cell cycle and signaling proteins. Previous findings show that head and neck cancer cells overexpress Crm1 and that these cells become highly dependent on Crm1 function. The results of this study show that after decreasing Crm1 expression levels in HNSCC cells through either treatment with specific Crm1 RNA interference (siRNA) or the selective Crm1 inhibitor leptomycin B (LMB), cell viability, proliferation, migration, and wound-healing abilities decreased, suppressing

• Sáng kiến kinh nghiệm
• Head and neck cancer
• Chromosome region maintenance 1
• RNA interference
• Leptomycin B
• Nuclear export signal
• BMC Cancer
• Head and neck squamous cell carcinoma
• Immune checkpoint inhibitors
• Survival in head and neck cancer
• Alcohol consumption
• Cigarette smoking
• Cohort studies
• Head neck cancer
• Head neck cancer subtypes
• Progressive resistance training
• Physical activity
• Body composition
• Clinical cohort
• Prognosis research
• Patient reported outcomes
• Sexual history
• Quality of life
• Biological samples
• Cell Line Microarray
• Potential immunohistochemical biomarkers
• Triple treatment combination
• Immunotherapy targeting
• Metastatic head
• Neck squamous cell carcinoma
• Cancer stem cells
• Side population
• Delayed neck metastasis
• Inflammatory marker
• Systemic inflammation
• Nonstandard treatment
• Patient compliance
• Cumulative incidence
• Esophageal cancer
• Metachronous cancer
• Young onset
• Head and neck
• Phase III
• Squamous cell carcinoma
• Overall survival
• Platinum therapy
• Narrow band imaging
• Second primary cancer
• Radiation dermatitis
• Topical steroid
• Neck cancer
• Patient derived xenograft
• Squamous cell cancer
• Anti cancer therapeutics
• Warburg effect
• Alternative splicing
• Pyruvate Kinase muscle isoenzyme
• Cancer cells
• Single nucleotide polymorphism
• Personalized medicine
• 5 lipoxygenase
• Representative endocannabinoids
• Effectively inhibited
• Treatment outcome
• Induction chemotherapy
• Neck squamous cells carcinomas
• Metabolic statuses
• CUP syndrome
• Delayed adjuvant therapy
• Advanced head and neck cancer
• Neck dissection
• Human papillomavirus
• Oropharyngeal cancer
• Laryngeal cancer
• Oral cancer
• Chemo radiotherapy exhibiting weak
• Locoregional cancer
• Aspiration pneumonia
• Risk factors
• Cancer related mortality
• Tumor growth
• Meta analysis
• Cancer worldwide
• Anti CD44 v6 antibody
• Antineoplastic treatment
• Indicating greater difficulties
• Circulating tumor cell
• Circulating tumor DNA
• Oral cavity cancer preclinical model