TAILIEUCHUNG - Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles

Cationic polyamidoamine (PAMAM) dendrimers are highly branched nanoparticles with unique molecular properties, which make them promising nanocarriers for gene delivery into cells. | Turkish Journal of Biology Research Article Turk J Biol (2018) 42: 63-75 © TÜBİTAK doi: Ultrasound-enhanced gene delivery to alfalfa cells by hPAMAM dendrimer nanoparticles 1 1 1, 2 1 Amin AMANI , Nasser ZARE *, Asadollah ASADI , Rasool ASGHARI-ZAKARIA Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran 2 Department of Biology, Faculty of Basic Sciences, University of Mohaghegh Ardabili, Ardabili, Iran Received: Accepted/Published Online: Final Version: Abstract: Cationic polyamidoamine (PAMAM) dendrimers are highly branched nanoparticles with unique molecular properties, which make them promising nanocarriers for gene delivery into cells. This research evaluated the ability of hyperbranched PAMAM (hPAMAM)-G2 with a diethylenetriamine core to interact with DNA, its protection from ultrasonic damage, and delivery to alfalfa cells. Additionally, the effects of ultrasound on the efficacy of hPAMAM-G2 for the delivery and expression of the gusA gene in the alfalfa cells were investigated. The electrophoresis retardation of plasmid DNA occurred at an N/P ratio (where N is the number of hPAMAM nitrogen atoms and P is the number of DNA phosphorus atoms) of 3 and above, and hPAMAM-G2 dendrimers completely immobilized the DNA at an N/P ratio of 4. The analysis of the DNA dissociated from the dendriplexes revealed a partial protection of the DNA from ultrasound damage at N/P ratios lower than 2, and with increasing N/P ratios, the DNA was better protected. Sonication of the alfalfa cells in the presence of ssDNA-FITC-hPAMAM increased the ssDNA delivery efficiency to 36%, which was significantly higher than that of ssDNA-FITC-hPAMAM without sonication. Additionally, the efficiency of transfection and the expression of the gusA gene were dependent on the N/P ratio and the highest efficiency .

• Sinh học
• Gene transfer
• hPAMAM–DNA complex
• Medicago sativa L
• Polyamidoamine dendrimers
• Gene delivery into cells
• BMC Genomics
• Horizontal gene transfer
• Lateral gene transfer
• Gene loss
• Rate variation
• Sperm mediated gene transfer
• Sperm mediated gene transfer in chicken
• Quality of spermatozoa
• DNaseI treatment
• Inhibitory factors of semen
• Acquired genes
• Mobile genetic elements
• Physical linkage
• Association analysis
• Linear mixed models
• Genome Biology
• Gene loss endosymbiotic gene transfer
• Organellar genome
• Biochemical complexity
• Bacterial progenitors
• BMC Bioinformatics
• Transfer learning
• Knowledge transfer
• Functional modules
• Functional mapping
• Classification rules
• Integrative modeling
• Gene expression
• BMC Plant Biology
• Intracellular gene transfer
• Meadow rue
• Plastid genome
• Gene tree
• Genetic algorithm
• Gene duplication
• Regulatory networks
• Dorsoventral patterning
• Lecture Microbiology
• Roles of microbes
• Biological research
• Genetic engineering
• Plasmid types
• Intergenomic gene transfer
• Mitochondrial genome
• Chloroplast genome
• Organellar genomes
• BMC Biotechnology
• Aggregated SCNT
• Abnormal gene expression
• Somatic cell nuclear transfer
• Donor cells
• Bayesian inference
• Molecular biologists
• Antibiotic resistance
• BMC Developmental Biology
• Foamy virus
• In vivo gene transfer
• Transferring exogenous genes
• Non mammalian models
• Phaseolus vulgaris
• Vertical gene transfer
• Comparative genomics
• Symbiosis genes
• Formica exsecta
• Transposable elements
• Intracellular accessory inhabitants
• Non specific lipid transfer proteins
• Gene family
• Expression profile
• Abiotic stress
• Genomic island
• Software tools
• Pierce’s disease
• Xylella fastidiosa
• Human genome
• Eukaryotic genomes
• Phylogenetic trees
• Chemical coupling
• Phage mediated gene transfer
• Gene delivery
• Transferrin targeted delivery
• AGS cell line
• Vibrio harveyi
• Common bean
• TAL effectors
• Host adaptation
• Algal virus
• Gene family evolution
• Plantfungal interactions
• Symbiont autonomy
• Long divided microbiologists
• Rampant hybridization
• Abundant gene flow