TAILIEUCHUNG - Chemical composition analysis and antibacterial-antiinflammatoryactivity tests of tamanu seed oil extracted by supercritcial fluid technology

The natural product of tamanu oil is valuable in medicine and cosmetic. In this study, the tamanu oil was extracted by supercritical CO2 (sCO2) and Soxhlet (using hexane solvent) methods. The fatty acids and coumarins compound of the obtained tamanu oil were analyzed by gas chromatography–mass spectrometry (GC-MS). The antibacterial and anti-inflammatory activities were also investigated by the diffusion method and performed on the white mice ears (Swiss albino). | TAÏP CHÍ PHAÙT TRIEÅN KH&CN, TAÄP 19, SOÁ K6- 2016 Chemical composition analysis and antibacterial-antiinflammatoryactivity tests of tamanu seed oil extracted by supercritcial fluid technology Nguyen Huu Hieu Tran Thi Minh Thuy Ho Chi Minh city University of Technology, VNU-HCM. (Manuscript Received on July 08th, 2016, Manuscript Revised September 03rd, 2016) ABSTRACT The natural product of tamanu oil is valuable in medicine and cosmetic. In this study, sCO2 was the highest ( µg/g), and times as much as that using Soxhlet method the tamanu oil was extracted by supercritical CO2 (sCO2) and Soxhlet (using hexane solvent) and the commercial oil, respectively. Besides, the results of investigation of the antibacterial methods. The fatty acids and coumarins activity suggested that the tamanu oil extracted compound of the obtained tamanu oil were analyzed by gas chromatography–mass using sCO2 was able to against some of strains namely: Staphylococcus aureus (S. aureus) spectrometry (GC-MS). The antibacterial and anti-inflammatory activities were also ATCC 25923, S. aureus ATCC 29213, methycilin-resistant S. aureus (MRSA) ATCC investigated by the diffusion method and performed on the white mice ears (Swiss 43300, and aeruginosa) albino). Content of fatty acid in tamanu oil In term of the anti-inflammatory activity, the extracted using sCO2 ( %) was higher than that using Soxhlet method. The compound of tamanu oil extracted using sCO2 was antiinflammatory effectively with reducing coumaron (2H-1-bezopyran-2-one, C9H6O2) was identified belonging the simple coumarin group flammatory: – % as for the thickness of mice ears and – % as and its content in the tamanu oil extracted using for the weight of mice ears. Pseudomonas ATCC aeruginosa (P. 277853. Keywords: Tamanu oil, coumarin, antibacterial, anti-inflammatory, extraction, supercritical CO2. Trang 145 SCIENCE & TECHNOLOGY DEVELOPMENT, Vol 19, .

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