TAILIEUCHUNG - Preparation and characterization of acellular porcine pericardium for cardiovascular surgery

The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. | Turkish Journal of Biology Turk J Biol (2016) 40: 1243-1250 © TÜBİTAK doi: Research Article Preparation and characterization of acellular porcine pericardium for cardiovascular surgery 1, 1 1, 1, 1 2 Ha Le Bao TRAN *, Trang Thi Huyen DINH **, My Thi Ngoc NGUYEN **, Quan Minh TO , Anh Tho Tuan PHAM Department of Physiology and Animal Biotechnology, Faculty of Biology–Biotechnology and Laboratory of Tissue Engineering and Biomedical Materials, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam 2 Cho Ray Hospital, Ho Chi Minh City, Vietnam Received: Accepted/Published Online: Final Version: Abstract: The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice. Key words: Pericardium, decellularization, patch, cardiovascular, biocompatibility 1. Introduction Cardiovascular diseases remain the leading cause of death for both men and women around the world. Such diseases killed approximately million people in 2012 (Mozaffarian et al., 2012). For patients, taking medication can help alleviate symptoms, but there are several possible side effects associated with medicine. Based on the fact that it is .

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