TAILIEUCHUNG - Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. | Turkish Journal of Biology Turk J Biol (2016) 40: 1227-1234 © TÜBİTAK doi: Research Article Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species 1 1 1 Carolina Mariane MOREIRA , Helena Botelho de ANDRADE , Suzan Kelly Vilela BERTOLUCCI , 2 3 1, Osmar Alves LAMEIRA , Aliyu MOHAMMED , José Eduardo Brasil Pereira PINTO * 1 Plant Tissue Culture and Medicinal Plants Laboratory, Department of Agriculture, Federal University of Lavras (UFLA), Lavras, Brazil 2 Genetic Resources and Biotechnology, Embrapa Amazônia Oriental, Belem, Brazil 3 Department of Biology, Faculty of Arts and Sciences, Abant İzzet Baysal University, Bolu, Turkey Received: Accepted/Published Online: Final Version: Abstract: The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction ( and , respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6trichloropicolinic acid (picloram; ), 2,4-D (40%), or TDZ (). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum and , respectively). A combination of TDZ and 2,4-D improved callus induction () but did not increase shooting response. However, both callusing and shooting .
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