TAILIEUCHUNG - Introduction To Animal Tissue Culture

Adherent monolayer on a solid substrate (various cell types) suspension in the culture medium (few cell types), A fragment of tissue attachment and migration occurs in the plane of the solid substrate, A spherical or three-dimensional shape specific histological interaction | Introduction to Animal Tissue culture Experimental Method in Medical Physiology 2006 1 What is tissue culture? In vitro culture (maintain and/or proliferate) of cells, tissues or organs. Types of tissue culture Cell culture Primary explant culture Organ culture 2 3 Cell culture: Adherent monolayer on a solid substrate (various cell types) suspension in the culture medium (few cell types) Primary explant culture: A fragment of tissue attachment and migration occurs in the plane of the solid substrate Organ culture: A spherical or three-dimensional shape specific histological interaction Three major categories of tissue culture Explant: living cells, tissues, or organs from animals or plants that transfer to a nutrient medium. Cell culture & Enzymatic Dissociation 4 Tissue from an explant is dispersed, mostly enzymatically, into a cell suspension which may then be cultured as a monolayer or suspension culture. Advantages & Disadvantages Advantages Development of a cell line over several generations Scale-up is possible Absolute control of physical environment Homogeneity of sample Less compound needed than in animal models Disadvantages Cells may lose some differentiated characteristics. Hard to maintain Only grow small amount of tissue at high cost Dedifferentiation Instability, aneuploidy 5 Tissue Culture Is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. 6 7 Advantages Some normal functions may be maintained. Better than organ culture for scale-up but not ideal. Disadvantages Original organization of tissue is lost. Advantages & Disadvantages Organ culture The entire embryos or organs are excised from the body and culture Advantages Normal physiological functions are maintained. Cells remain fully differentiated. Disadvantages Scale-up is not recommended. Growth is slow. Fresh explantation is required for every experiment. 8 EMP04 9 Why do we

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