TAILIEUCHUNG - Some optimal cultural parameters for gibberellic acid biosynthesis by Pseudomonas sp.
The current study reports the optimal cultural parameters for GA3 production by Pseudomonas sp. isolated from wastes of processed olive affected by physiological conditions (incubation temperature, pH of the growth media, incubation period, and incubation conditions). | Turk J Biol 30 (2006) 81-85 © TÜB‹TAK Some Optimal Cultural Parameters for Gibberellic Acid Biosynthesis by Pseudomonas sp. 1 2 fiafak KARAKOÇ , Nilüfer AKSÖZ 1 Ministry of Agriculture and Rural Affairs, General Directorate of Agricultural Researches, Istanbul Road, 06171 Yenimahalle, Ankara - TURKEY 2 Department of Biology/Biotechnology, Faculty of Science Hacettepe University, Ankara - TURKEY Received: Abstract: The current study reports the optimal cultural parameters for GA3 production by Pseudomonas sp. isolated from wastes of processed olive affected by physiological conditions (incubation temperature, pH of the growth media, incubation period, and incubation conditions). The highest level of gibberellic acid ( mg/l) production was obtained in nutrient broth when the bacteria culture was incubated at 30 °C for 72 h at pH 7 on rotary shaker and in the dark. Key Words: Plant growth regulators, Gibberellic acid, Pseudomonas sp., G. fujikuroi, Processed olive waste Pseudomonas sp.’ den Gibberellik Asit Sentezi ‹çin Baz› Optimal Kültürel Parametreler Özet: Bu çal›flmada fizyolojik koflullarca (inkübasyon s›cakl›¤›, besi ortam› pH’› ve baz› inkübasyon koflullar›) etkilenen ya¤ fabrikas› at›klar›ndan izole edilmifl Pseudomonas sp. taraf›ndan Gibberellik asit üretimi için optimal kültürel parametreler bildirilmektedir. En yüksek Gibberellik asit üretim seviyesi (285,06 mg/l), 30 °C’de, 72 saat, pH 7’de çalkalamal› shaker’da ve karanl›kta nutrient broth’daki bakteri kültüründe elde edilmifltir. Anahtar Sözcükler: Bitki büyüme regülatörleri, Gibberellik asit, Pseudomonas sp., G. fujikuroi, Ya¤ fabrikas› at›¤› Introduction Plant growth regulators such as gibberellins and cytokinins are important biotechnological and economical products. They are commonly used in agriculture, viticulture, gardens and horticulture (1). Gibberellins (GAs) are a large group of important diterpenoid acids among commercial phytohormones (2). They are endogenous .
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