TAILIEUCHUNG - Identification of lactococcus lactis subsp. isolates based on their cell wall protein profiles and plasmid contents

In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization. | Turk J Biol 33 (2009) 21-27 © TÜBİTAK doi: Identification of Lactococcus lactis subsp. Isolates Based on Their Cell Wall Protein Profiles and Plasmid Contents Mehtap ÜSTÜN1, Banu ÖZDEN1, Ömer ŞİMŞEK2, Mustafa AKÇELİK1 1 Ankara University, Faculty of Science, Department of Biology, Tandoğan, Ankara - TURKEY 2 Ankara University, Institute of Biotechnology, Beşevler, Ankara - TURKEY Received: Abstract: In this study, among the total 50 lactococci isolates, 44% L. lactis subsp. lactis, 28% L. lactis subsp. lactis biovar. diacetylactis and 28% L. lactis subsp. cremoris strains were identified by their phenotypic characterization. According to the cell wall protein patterns, , , , , , , and kDa proteins were found identical within 82% of isolates. Moreover 93% of L. lactis subsp. cremoris strains contained a unique kDa protein; however, L. lactis subsp. lactis and L. lactis subsp. lactis biovar. diacetylactis had no unique protein in their groups. Also a key plasmid profile, which would be used to determine the isolates at subspecies level, could not be obtained. Key Words: L. lactis, cell wall proteins, plasmid Hücre Duvarı Protein Profilleri ve Plazmid İçerikleri Esas Alınarak Lactococcus lactis subsp. İzolatlarının Tanımlanması Özet: Bu çalışmada, fenotipik karakterizasyonu yapılan 50 adet laktokok izolatının, % 44’ü L. lactis subsp. lactis, % 28’i L. lactis subsp. lactis biovar. diacetylactis ve % 28’i L. lactis subsp. cremoris olduğu belirlendi. Hücre duvarı protein profili neticesinde izolatların % 82’sinde 8,6, 12,5, 14,7, 20,3, 23,4, 25,4 ve 31,8 kDa moleküler büyüklükteki proteinlerin ortak olduğu bulundu. L. lactis subsp. lactis ve L. lactis subsp. lactis biovar. diacetylactis suşları ayırıcı nitelikte proteine sahip değilken, L. lactis subsp. cremoris suşlarının % 93’ü 157,8 kDa büyüklükte farklı bir protein içerdi. Suşların alt tür düzeyinde belirlenmesinde kullanılabilecek anahtar plazmid

• Sinh học
• lactis
• Cell wall proteins
• Phenotypic characterization
• Polyacrilamide gel electrophesis
• Sodium dodecyl sulphate
• Trichloroacetic acid