TAILIEUCHUNG - The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX)
This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA3), from stage 8 to 11, for 96 h under static renewal test conditions. | Turk J Biol 33 (2009) 181-188 © TÜBİTAK doi: The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX) Ayper BOĞA1, Seçil BİNOKAY1, Yaşar SERTDEMİR2 1Department of Physiology, Faculty of Medicine, Çukurova University, Adana - TURKEY 2Bioistatistics, Faculty of Medicine, Çukurova University, Adana - TURKEY Received: Abstract: This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA3), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC50), malformation (EC50), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC50 and EC50 values determined for GA3 exposure were mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA3 was . Different anomalies occurred in the embryos, depending on the GA3 concentration. Based on these results, we conclude that GA3 is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture. Key words: Gibberellic acid, FETAX, Teratogenicity, Toxicity, Xenopus laevis Gibberellik asit’in (GA3) toksisite ve teratojenitesinin kurbağa embriyo teratogenezis testi (FETAX) kullanılarak değerlendirilmesi Özet: Çalışmada, bitki büyüme düzenleyicisi gibberellik asitin (GA3) (bir pestisit tipi) potansiyel gelişimsel toksisitesi FETAX testi kullanılarak değerlendirildi. Xenopus laevis embriyoları (evre 8-11); 96 saatlik statik yenileme test koşullarında, gibberellik asitin 11 konsantrasyonuna maruz bırakıldı. Muamelenin sonucunda, ortalama letal konsantrasyon
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